工程UDP-GlycosyltransferaseUGTPg29用于从Protopanaxadiol中有效合成金赛诺化物Rg3
Huichang He1, Jiajie Chen1, Jiangtao Xie1
1College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, 211816, China.
Applied biochemistry and biotechnology
|August 9, 2024
概括
研究人员设计了酶,以有效地从原型帕纳克萨迪醇 (PPD) 产生罕见的Rg3和Rh2人参化物. 这种优化的"一子"酶系统实现了高产的金氏化物Rg3,为工业生物合成提供了潜力.
科学领域:
- 生物技术是生物技术.
- 酶学 是一种酶学.
- 自然产品的合成自然产品的合成
背景情况:
- 罕见的Rg3和Rh2人参化物具有显著的药理特性.
- 这些化合物是protopanaxadiol (PPD) 衍生物,通过UDP-glycosyltransferases进行酶合成.
研究的目的:
- 为了设计UDP-glycosyltransferases,以提高Rg3和Rh2.2. ginsenosides的产生.
- 为了开发一个高效的单酶系统,用于人参酸Rg3生物合成.
主要方法:
- 细菌细菌的突变性UDP-糖转移酶 (Bs-YjiCm) 和帕纳克斯人参的半理性设计 UGTPg29.
- 在一反应系统中将工程酶与糖糖合成酶结合起来.
- 反应条件的优化和料批量策略的实施.
主要成果:
- 在Bs-YjiCm中发生的N178I突变增加了Rh2的产生.
- 改造后的UGTPg29变种 (R91M/D184M/A287V/A342L) 显示出更好的稳定性和活性.
- 一个三酶系统在24小时内实现了12.38mM (9.72g/L) 的高Rg3产量.
结论:
- 优化的酶系统使人参酸Rg3.3的高效生物合成成为可能.
- 工程UDP-glycosyltransferases是工业性金氏化物生产的有希望的候选者.
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