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通过将工程生物合成和储存途径与高光处理相结合,提高叶子中的亲维生素A含量和生物可访问性

Luca Morelli¹, Pablo Perez-Colao¹, Diego Reig-Lopez¹

¹Institute for Plant Molecular and Cell Biology (IBMCP), CSIC-Universitat Politècnica de València, Valencia, 46022, Spain.

The Plant journal : for cell and molecular biology

|August 9, 2024

概括

用β-胡卜素 (β-胡卜素) 生物强化蔬菜是一项挑战. 结合细胞质生产 (策略C) 与塑转化 (策略P) 和强光,显著提高了β-胡卜素及其在植物叶中的生物利用性.

科学领域:

植物生物技术植物生物技术
营养科学营养科学
农业科学农业科学

背景情况:

叶蔬菜的生物强化用维生素A类卡洛,如β-卡洛,面临着重大障碍.
优化胡卜素水平而不会对光合作用产生负面影响,对于成功的生物强化至关重要.

研究的目的:

开发和增强提高可食用叶子中β-胡卜素积累和生物可用性的策略.
研究基因工程和环境因素对胡卜素含量的联合影响.

主要方法:

采用了两种策略:细胞质生产β-胡卜素 (策略C) 和使用细菌植物合成酶 (策略P) 将叶绿体转化为染色体.
利用了对策略P的crtB编码结构的农业透/感染,确保非工程叶子支持植物生长.
应用增加光强度的处理以促进塑球蛋白的增殖和增强β-胡卜素的生物可访问性.

主要成果:

策略C和策略P的组合增加了Nicotiana benthamiana叶中的β-胡卜素水平的五倍.
通过强烈的光处理促进塑球蛋白增殖,以及C和P策略,导致可访问β-胡卜素增加30倍.
仅仅较高的光强度和策略P也提高了叶 (*Lactuca sativa*) 的β-胡卜素含量和生物可访问性.

结论:

结合细胞质生产和塑转换的双重方法,加上优化的光照条件,对于β-胡卜素生物强化非常有效.
刺激塑球蛋白的增殖是增强β-胡卜素积累和其在食用植物中的生物可用性的关键因素.
这种综合战略显示,通过提高亲维生素A含量,有望提高主食作物的营养价值.

关键词:

生物可访问性生物可访问性生物强化生物强化胡卜素是一种胡卜素.菜菜菜菜塑料球体是一种塑料球体.维生素A是一种维生素A.在β-胡卜素β-胡卜素.

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