喷雾化电子液体基成分诱导了口服角质细胞中细胞毒性和基因毒性
Tao Ma1, Angela Lee2, Bryan Eng2
1Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, Maryland, USA.
Oral diseases
|August 9, 2024
概括
电子烟气溶从甘醇/甘 (PG/G) 电子液体中产生,会对口腔角质细胞造成显著的细胞损伤和DNA损伤. 这凸显了电子烟的风险,即使没有尼古丁或风味.
科学领域:
- 口服毒理学 口服毒理学
- 细胞生物学 细胞生物学
- 电子烟研究的研究.
背景情况:
- 电子烟 (e-cigarette) 的使用正在增加,引发了对口腔健康影响的担忧.
- 电子烟气溶含有有害和潜在有害成分 (HPHC).
- 烯基醇/甘油 (PG/G) 电子液体气溶对口腔角质细胞的特定影响尚不清楚.
研究的目的:
- 为了研究无尼古丁,无味的PG/G电子液体气溶对正常口腔角质细胞的毒理作用.
- 要确定气溶化过程本身是否有助于细胞毒性.
主要方法:
- 口服角质细胞细胞系 (NOKSI) 暴露以模拟电子液体气溶和非气溶对照.
- 检测细胞活力,细胞毒性和基因毒性.
- 与已知HPHC在电子烟气溶中发现的acrolein进行比较.
主要成果:
- 与非气溶液电子液相比,PG/G电子液气溶提取物在NOKSI细胞中显著增加了细胞毒性.
- 气溶暴露导致了口腔角质细胞中显著的DNA损伤反应.
- 暴露于阿克罗莱因产生了类似的细胞毒性和基因毒性作用.
结论:
- PG/G电子液体的空气化是诱导口腔角质细胞中细胞毒和基因毒作用的关键因素.
- 蒸汽PG/G电子液体,即使没有尼古丁或风味,也对口腔组织健康构成风险.
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