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提高酶催化效率:通过优化外部表面利用,在HHSS上固定乳酶以实现协同双A吸附和生物降解

Hongxia Yu¹, Lijun Feng¹, Mohamed Abbas²

¹School of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China.

International journal of biological macromolecules

|August 9, 2024

概括

这项研究提出了一种新型的等级空心球 (HHSS) 用于固定乳糖酶,显著提高酶负载和双A (BPA) 降解效率. 由此产生的HHSS-LE生物催化剂显示出优异的可回收性和比传统材料更优异的性能.

科学领域:

生物催化剂是一种生物催化剂.
材料科学材料科学材料科学
环境化学环境化学

背景情况:

乳糖酶有效降解像双甲 (BPA) 这样的化合物.
对于laccase的常规固定方法通常会受到低酶负荷和降低的催化效率的影响.
优化外部表面利用对于提高生物催化剂性能至关重要.

研究的目的:

开发一种高效的方法,以使用氨基功能化的层次空心体球 (HHSS) 来进行乳酸酶固定.
研究HHSS吸附和乳糖酶降解对增强BPA去除的协同效应.
使用盒子-贝恩肯设计 (BBD) 和响应表面方法 (RSM) 来优化固定条件.

主要方法:

氨基功能化HHSS的合成用于laccase固定.
在HHSS上交叉连接laccase以创建HHSS-LE生物催化剂.
应用BBD和RSM以优化酶负载和反应条件.

主要成果:

在HHSS.上达到高达213.102 mg/g的高酶负载.
在50-200 mg/L范围内,已证明BPA清除效率优越,超过100%.
与MCM-41和固体二氧化球 (SSS) 相比,HHSS表现出更高的酶负载能力和催化活性.
在11个循环中,HHSS-LE生物催化剂显示出了显著的可回收性.

结论:

层次空洞的二氧化球为酶固定提供了卓越的外部表面利用.
开发的HHSS-LE生物催化剂为BPA降解提供了一种高效和可回收的解决方案.
这种方法为设计普遍适用的酶固定催化剂奠定了基础.

关键词:

吸附-生物降解的协同作用在BBD-RSM中使用.拉卡塞斯固定运动

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