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不同的方法来理解在生态生理学研究中对小生态热虫的方法论的充分性

Rafaella Silva Roseno¹, Leildo Machado Carilo Filho², Luisa Maria Diele-Viegas³

¹Graduate Program in Zoology, Universidade Estadual de Santa Cruz, Rodovia Jorge Amado, Km 16, 45662-900 Salobrinho, Ilhéus, Bahia, Brazil; Tropical Herpetology Laboratory, Department of Biological Sciences, Universidade Estadual de Santa Cruz, Rodovia Jorge Amado, Km 16, 45662-900 Salobrinho, Ilhéus, Bahia, Brazil; Laboratory of (Bio)diversity in the Anthropocene, Biology Institute, Universidade Federal da Bahia, 1154, R. Barão de Jeremoabo, 668, 40170-115 Ondina, Salvador, Bahia, Brazil.

Comparative biochemistry and physiology. Part A, Molecular & integrative physiology

|August 9, 2024

概括

精确测量小的临界热极限对于评估它们的脆弱性至关重要. 使用红外温度计的基板温度协议最能反映的体温,以便进行可靠的生态生理学研究.

科学领域:

生态生态学生态生态学
生理学生理学生理学
类学类学类学类学

背景情况:

由于它们与环境的密切关系,小型外热生物表现出强烈的体温 (Tb) 变化.
方法上的差异可能会影响测量小的热生理学,影响脆弱性评估.

研究的目的:

研究不同测量技术如何影响小Coleodactylus meridionalis的临界热最大值 (CTmax) 和临界热最小值 (CTmin).
建立一个方法上的共识,对生态生理学研究小生态热生物.

主要方法:

四种温度测量方案进行了比较:直接的中体Tb,室温,基板温度和使用升级试验的Tb.
红外温度计被用来测量基板和体温.
使用C. meridionalis的116个个体,进行了177个CTmax和131个CTmin测量.

主要成果:

当通过Tb.直接测量时,Coleodactylus meridionalis的平均CTmax为41°C,CTmin为8.9°C.
基板温度协议非常接近的实际体温 (Tb).
对于小,使用红外温度计的基板温度方法是建议的.

结论:

方法选择显著影响小的热生理学测量.
使用红外温度计的基板温度协议为像C. meridionalis.这样的小型外热生物提供了最可靠的CTmax和CTmin估计.
准确的热量数据对于预测物种对环境变化的脆弱性至关重要.

关键词:

气候变化气候变化气候变化临界温度是指关键温度.生态生理学生态生理学方法论方法论方法论它们是Sphaerodactylidae的一类.

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