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亚细胞分离和元蛋白基因组识别和验证基质细胞疾病的关键差异表达分子标

Elvis B Kidzeru¹, Musalula Sinkala², Temwani Chalwa³

¹MRC-SA Wound Healing and Keloid Research Unit, Division of Dermatology, Department of Medicine, Groote Schuur Hospital, University of Cape Town, Cape Town, South Africa; Microbiology, Infectious Diseases, and Immunology Laboratory (LAMMII), Centre for Research on Health and Priority Pathologies (CRSPP), Institute of Medical Research and Medicinal Plant Studies (IMPM), Ministry of Scientific Research and Innovation, Yaoundé, Cameroon; Division of Radiation Oncology, Department of Radiation Medicine, Groote Schuur Hospital, Faculty of Health Science, University of Cape Town, Cape Town, South Africa.

The Journal of investigative dermatology

|August 9, 2024

概括

使用蛋白质组学和转录组学识别了状腺疾病 (KD) 生物标志物NF-kB-p65 (RELA) 和CAPN2. 这些发现可能通过向细胞亡来改善 keloid 诊断和治疗方法.

科学领域:

生物化学生物化学
分子生物学分子生物学
皮肤病学皮肤病学

背景情况:

状体疾病 (KD) 是一种纤维扩散性疾病,其病因不明,治疗选择有限.
状痕是局部侵略性的,侵入性的病变,尽管是良性的.
目前从蛋白质和基因组研究中对KD生物标志物的理解是有限的.

研究的目的:

为了确定新的致病候选人和生物标志物,为 keloid 疾病.
对正常和KD纤维细胞进行全面的蛋白质组学和转录组学分析.
利用综合生物信息学来发现生物标志物.

主要方法:

对亚细胞分数和全细胞溶解物的综合蛋白质组学分析.
对正常与KD纤维细胞的转录学数据分析.
综合生物信息学,流细胞计,免疫组织化学和实时细胞分析.

主要成果:

在KD纤维细胞中,NF-kB-p65 (RELA) 和CAPN2显著上调.
通过流细胞计和免疫组织化学证实了RELA和CAPN2的升级.
奥梅普拉和甲通过增强亡来抑制KD纤维细胞的生长.

结论:

NF-kB-p65 (RELA) 和CAPN2是 keloid 疾病的新型,重要的生物标志物.
这些生物标志物与改变的信号通路有关,包括亡.
亚细胞分离和元蛋白基因组分析为KD诊断和治疗提供了新的途径.

关键词:

在CAPN2的基础上,CAPN2是CAPN2.凯洛因体疾病是什么?俄梅普拉 (Omeprazole) 是一种药物.蛋白质组学是指蛋白质组学.在RELA中,您可以使用RELA.

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