克鲁兹杆菌聚集了宿主细胞质处理体,以逃避天生的免疫反应
Eri Seto1, Shinichiro Kina2, Reika Kawabata-Iwakawa3
1Education and Research Support Center, Gunma University Graduate School of Medicine, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan.
杆菌感染促进处理体 (PB) 积累以抑制宿主天生的免疫力. 通过EDC4淘汰破坏PB组装可以增加IL-1β的产生,从而揭示了寄生虫的免疫逃避策略.
科学领域:
- 细胞生物学 细胞生物学
- 寄生虫学的寄生虫学
- 免疫学 免疫学 免疫学
背景情况:
- 处理体 (PBs) 是参与mRNA调节的细胞质焦点.
- 一种Trypanosoma cruzi感染诱导了PB在宿主细胞中的积累.
- 在宿主-寄生虫相互作用中PBs的作用基本上是未知的.
研究的目的:
- 在T. cruzi感染期间调查PBs在宿主mRNA代谢中的作用.
- 为了确定受T. cruzi. 影响的PB调节宿主基因.
- 阐明T. cruzi操纵宿主PBs的机制.
主要方法:
- 建立了一个EDC4淘汰人类纤维瘤细胞系,以创建PB缺陷细胞.
- 利用下一代测序来对野生类型和淘汰细胞的转录组概况.
- 分析了基因表达的变化和IL-1β的蛋白质水平.
主要成果:
- 在EDC4KO细胞中,PB的枯竭导致了先天免疫信号通路的上调.
- 在T. cruzi感染后,PB缺乏细胞中,炎症前的细胞因子IL-1β显著增加.
- 恢复PB组件抑制了IL-1β表达,而突变EDC4则没有这样做.
结论:
- T. cruzi感染促进宿主PB组装以抵消抗寄生虫天生的免疫力.
- 在T. cruzi感染期间,PBs在调节宿主炎症反应中发挥着至关重要的作用.
- 准PB组装可能是一个潜在的策略,以增强宿主免疫力T. cruzi.
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