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改造的细菌膜囊泡作为安全高效的纳米加热器来重新编程瘤微环境,以增强免疫疗法

Kunguo Liu¹, Shiyu Du², Jiawei Yang¹

¹The Second Affiliated Hospital of Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.

Journal of controlled release : official journal of the Controlled Release Society

|August 9, 2024

概括

研究人员开发了合成细菌囊泡 (SBVs),以克服癌症治疗的外部膜囊泡 (OMVs) 的低产量. 这些SBV通过提供催化酶和激活免疫路径来增强免疫疗法,提供一种强效和安全的治疗策略.

科学领域:

免疫学免疫学免疫学
生物技术是生物技术.
材料科学材料科学材料科学

背景情况:

免疫抑制性瘤微环境 (TME) 阻碍了免疫治疗在固体瘤中的有效性.
细菌外膜囊泡 (OMVs) 对癌症疫苗有希望,但受益率低.
需要一种新的方法来增强OMV的生产和治疗潜力.

研究的目的:

开发一种高产量的合成细菌囊泡 (SBV) 平台,用于癌症治疗.
为增强免疫激活和瘤微环境调节设计SBV.
将免疫疗法和光疗结合起来,以获得协同作用的抗瘤效应.

主要方法:

通过安培西林预处理和细菌的超声波合成SBV,比OMV提高了40倍的产量.
在SBV中封装的催化酶 (CAT) 缓解瘤缺氧,并激活cGAS/STING通路.
装饰的SBV配有聚多巴胺 (PDA) ,以减轻脂聚糖 (LPS) 毒性,并装载了用于光疗的绿色氨酸 (ICG).

主要成果:

与天然的OMV相比,SBV的收益率明显高.
SBVs有效地提供了催化酶,缓解了瘤缺氧,并激活了cGAS/STING免疫通路.
通过SBV@PDA/ICG平台,通过联合免疫疗法和光疗法,降低毒性,显示出协同作用的抗瘤效应.

结论:

SBVs代表了癌症免疫治疗的可扩展和多功能平台.
该SBV@PDA/ICG系统提供了一个有前途的策略,用于重编程TME并增强抗瘤反应.
这种方法协同结合了免疫疗法和光疗法,用于安全和有效的癌症治疗.

关键词:

抗瘤免疫疗法免疫疗法细菌膜囊泡是一种细菌.瘤是一个瘤.瘤微环境是一个微环境.cGAS-STING 的使用情况.

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