在高血糖环境下HL-60细胞中性友分化的代谢特征
Jorge Andrés Cázares-Preciado1, Alejandra López-Arredondo1, José Antonio Cruz-Cardenas1
1Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, Nuevo Leon, Mexico.
BMJ open diabetes research & care
|August 9, 2024
概括
高葡萄糖会通过在分化过程中改变新陈代谢来损害中性粒细胞的功能. 这项研究显示,在高葡萄糖中,酸盐合成酶和卡尼丁棕甲基转移酶1活性下降,影响中性粒细胞样细胞的发育.
科学领域:
- 免疫学和新陈代谢
- 细胞生物学 细胞生物学
- 血液学 血液学 血液学
背景情况:
- 慢性高血糖会对中性粒细胞功能产生负面影响,减少杀死病原体的能力,增加发病率.
- 高血糖影响中性粒细胞代谢和骨髓分化的确切机制尚不清楚.
研究的目的:
- 在高葡萄糖条件下研究中性粒细胞的代谢和分化变化.
- 利用人类白血病细胞系 (HL-60) 模型,研究不同葡萄糖度的中性粒细胞样细胞分化.
主要方法:
- 使用高分辨率呼吸计和流动细胞计来分析供体中性粒细胞和HL-60细胞.
- 在标准和高葡萄糖介质中,HL-60细胞分化为中性粒细胞类细胞.
- 测量了氧气消耗率 (OCR),表面标记物表达 (CD15,CD11b) 和酶活性 (CPT1,酸合成酶).
主要成果:
- HL-60细胞分化显示了CD15和CD11b表达的增加,更高的细胞化和线粒体质量损失.
- 在高葡萄糖中分化的中性粒细胞样细胞表现出增加的CD11b表达和增强的细胞化.
- 在分化过程中,较高的葡萄糖度与酸盐合成酶和卡尼丁棕甲基转移酶1 (CPT1) 活性下降相关.
结论:
- HL-60分化模型有效地回顾了人类中性粒细胞分化特征.
- 葡萄糖度增加模仿高血糖性中性粒细胞表型,包括CD11b升高和细胞化.
- 这项研究首次证明了中性粒细胞类细胞中CPT1和酸合成酶活性在高葡萄糖下下调,独立于线粒体质量.
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