无标签,无接触的静止膜电位的测定,使用介电泳
Michael Pycraft Hughes1,2, Krista S P Clarke3, Rashedul Hoque3
1Department of Biomedical Engineering and Biotechnology, Khalifa University of Science and Technology, Abu Dhabi, UAE. Michael.hughes@ku.ac.ae.
Scientific reports
|August 9, 2024
概括
我们开发了一种新的非接触式方法,用于测量细胞静止膜电位 (RMP),使用介电泳. 这种无标签的技术是准确的,低成本的,非破坏性的,为传统的RMP测量方法提供了替代方案.
科学领域:
- 细胞电生理学 细胞电生理学
- 生物物理学的生物物理.
- 生物技术是生物技术.
背景情况:
- 细胞静止膜潜力 (RMP) 对于理解离子通道功能和细胞调节至关重要.
- 现有的RMP测量方法 (补丁,微电极,光体) 是昂贵的,缓慢的,取决于操作者,并且经常具有破坏性.
研究的目的:
- 引入一种非接触式,无标签的方法来估计细胞膜潜力.
- 为了验证这种基于介电泳的新方法在多种细胞类型中的准确性和适用性.
主要方法:
- 利用电介电泳来确定细胞质导电性斜率作为介质导电性的函数,用于膜电位估计.
- 结果与七种不同细胞类型的现有补丁数据进行了比较,包括初级和培养细胞以及癌症细胞系.
- 分析了离子通道抑制剂对膜电位测量的影响.
主要成果:
- 在七种不同的细胞类型 (红细胞,血小板,THP-1,冠状细胞,MSCs,HeLa,Jurkat) 中证明了介电泳法的准确性.
- 成功测量了制药剂 (TEA,DMSO,神经aminidase) 对膜潜力的影响.
- 与补丁数据相比,在公布的误差范围内实现了准确性.
结论:
- 开发的介电泳法为RMP测量提供了一个低成本,非破坏性,独立于操作者和无标签的替代方案.
- 这种技术允许细胞在测量后恢复,比传统方法具有显著的优势.
- 该方法准确地估计了膜潜力,并可以检测药理学剂的作用.
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