单细胞转录组分析显示,在患有骨关节结核病的患者中,巨-RGS1子集的频率下降
Ying Jiang1, Xinqiang Zhang2, Bo Wang1
1The Eighth Medical Center of Chinese PLA General Hospital, Beijing, 100091, People's Republic of China.
Molecular medicine (Cambridge, Mass.)
|August 9, 2024
概括
在骨关节结核病 (OTB) 中,一个特定的巨细胞子集 (巨细胞-RGS1高) 减少. 这个子组可以帮助诊断OTB并监测治疗有效性.
科学领域:
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 免疫细胞子集在Mycobacterium结核病 (MTB) 感染中发挥作用.
- 参与骨关节结核病 (OTB) 的特定免疫细胞子集尚不清楚.
- 单细胞RNA测序 (scRNA-Seq) 可以阐明免疫细胞的表型和功能.
研究的目的:
- 用scRNA-Seq.研究OTB患者免疫细胞子集的表型和功能.
- 确定OTB诊断和治疗监测的潜在生物标志物.
主要方法:
- scRNA-Seq是在OTB患者的病态和健康的邻近组织上进行的.
- 无监督集群和统一的多元近似和投影 (UMAP) 用于细胞子集的识别和可视化.
- 免疫组织化学和流细胞测量被用来验证OTB,骨关节细菌感染 (OBI) 和健康对照 (HC) 样本中的发现.
主要成果:
- 在OTB组织中发现了13个不同的细胞子集.
- 在OTB组织中,巨细胞-RGS1高子集的频率显著下降,与通过铁亡的MTB易感性增加相关.
- 在OTB组织和血液样本中降低了巨细胞-RGS1高子集频率,使OTB患者与HC和OBI患者有区别.
结论:
- 大细胞-RGS1高子集在OTB患者中减少,并且可能随着有效治疗而增加.
- 大细胞-RGS1高子集显示了作为OTB的诊断生物标志物的潜力.
- 这个子组也可以作为监测OTB治疗疗效的生物标志物.
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