在Bacillus subtilis BSNK-5发酵大豆奶中特征性风味成分的蛋白质组分析
Miao Hu1, Jiao Wang1, Yaxin Gao1
1Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, No. 2 Yuan Ming Yuan West Road, Beijing 100193, China.
Foods (Basel, Switzerland)
|August 10, 2024
概括
细菌细菌发酵可以改善大豆奶,但会产生独特的味道. 这项研究确定了负责这种特征性风味的关键蛋白质和途径,为改善发酵大豆食品提供了见解.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 细菌细菌发酵可以提高大豆奶的营养价值和生物可用性.
- 然而,B. subtilis产生的独特风味特征阻碍了其广泛的工业应用.
研究的目的:
- 为了识别导致B. subtilis BSNK-5发酵大豆奶特有的风味的关键蛋白质.
- 阐明了形成风味的基础分子机制.
主要方法:
- 协同质量标签 (TMT) 蛋白质组学被用来分析蛋白质表达.
- 进行了基因本体学 (GO) 和基因和基因组的京都百科全书 (KEGG) 丰富分析.
- 代谢学数据被整合,以确定关键的风味成分和途径.
主要成果:
- 总共有765种不同表达的蛋白质被确定.
- 通过丰富分析,70种蛋白质与特征性风味有关.
- 确定了15个关键蛋白酶和5个主要的风味形成途径,包括氨的产生,皮拉津的合成和短链脂肪酸的形成.
结论:
- 本研究提供了B. subtilis发酵大豆奶口味的全面蛋白质和代谢分析.
- 确定了关键蛋白质和途径,为减轻发酵大豆产品中不良风味提供了理论基础.
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