原型类化合物减轻脂聚糖诱导的肠道炎症,调节小鼠肠道微生物群
Jialu Huang1,2, Meishan Yue1,2, Yang Yang2
1College of Veterinary Medicine, Shanxi Agricultural University, Jinzhong 030801, China.
Animals : an open access journal from MDPI
|August 10, 2024
概括
在小鼠肠道炎症模型中,马克莱亚带状类化合物 (MPTA) 显示出治疗效果. MPTAs改善了肠道健康,减少了炎症标志物,并恢复了肠道微生物群平衡.
科学领域:
- 药理学 药理学是指药理学的学科.
- 胃肠病学 胃肠病学
- 微生物学 微生物学
背景情况:
- 肠道炎症是一个重要的健康问题.
- 脂多糖 (LPS) 是肠道炎症的强有力的诱导剂.
- 正在探索自然化合物的治疗潜力在炎症条件下.
研究的目的:
- 为了评估马克莱亚·科尔达塔 (Willd) 的治疗效果. 在小鼠的脂聚糖化物 (LPS) 诱导的肠炎上,R. Br. 衍生型原蛋白类类化合物 (MPTA) 的作用.
- 研究MPTAs对肠道形态,杯状细胞丰富度,炎症性细胞因子水平和肠道微生物群组成的影响.
主要方法:
- 动物模型:小鼠的LPS诱导的肠炎.
- 组织学分析:血素和乙素 (H&E) 和周期性酸-Schiff (PAS) 染色用于肠道形态和杯状细胞.
- 生物化学试验:ELISA检测血清中细胞因子 (IL-1β,IL-6,IL-8,TNF-α).
- 分子分析:用于mRNA表达的定量PCR (qPCR) (TLR4,NF-κB p65,NLRP3,IL-6,IL-1β) 和用于蛋白质水平的西部斑点 (TLR4,Md-2,MyD88,NF-κB p65,NLRP3).
- 微生物群分析: 16S rDNA 测序.
主要成果:
- 根据剂量,MPTAs治疗改善了肠道形态和增加了杯状细胞的丰度.
- MPTAs显著降低了促炎性细胞因子 (IL-1β,IL-6,IL-8,TNF-α) 的血清水平.
- MPTAs降低了关键炎症通路蛋白的表达,包括TLR4,MyD88,NLRP3和NF-κB p65.
- 高剂量MPTA治疗 (24 mg/kg) 恢复了肠道微生物的组成.
结论:
- 在治疗肠道炎症方面,MPTA具有显著的治疗潜力.
- MPTAs通过调节炎症信号通路和改善肠道屏障功能来发挥其作用.
- 在炎症性肠道疾病中,MPTA是一种有前途的天然治疗剂.
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