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来自Chimonanthus praecox的新型简单序列重复 (SSR) 标记物的建立转录组数据及其在品种识别中的应用

Bin Liu¹, Hua-Feng Wu¹, Yin-Zhu Cao¹

¹Chongqing Engineering Research Center for Floriculture, Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education), College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China.

Plants (Basel, Switzerland)

|August 10, 2024

概括

这项研究开发了新的分子标记物,用于识别传统的中国花树,Chimonanthus praecox.的品种. 这些表达序列标记-简单序列重复 (EST-SSR) 标记有效地区分了12种品种,有助于遗传多样性探索.

科学领域:

植物遗传学和育种.
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

奇蒙 (Chimonanthus praecox) 是一个重要的中国传统开花树,有许多品种,但只有很少有人正式命名.
准确识别C. praecox品种对于繁殖和保护工作至关重要.
表达式序列标记-简单序列重复 (EST-SSR) 标记对于植物品种识别有价值.

研究的目的:

开发和描述新型的EST-SSR标记物用于Chimonanthus praecox.
评估这些标记物对于区分C. praecox品种的有用性.
为基因多样性研究和C. praecox.的基因发现提供分子工具.

主要方法:

使用RNA测序 (RNA-seq) 来组装来自C. praecox.的162,638个单基因.
生物信息分析从11,691个单基因中确定了13,556个简单序列重复 (SSR) 位点.
设计了64,440个潜在的SSR原始对,其中75个对放大和多态性进行了测试;分析了12种C. praecox品种.

主要成果:

总共有13,556个SSR位点被确定,其中三核酸重复是最常见的.
七对EST-SSR原料表现出高多态性,并放大了预期大小的碎片.
这些标记物成功地将12种C. praecox品种聚集成两个不同的单类群.

结论:

C. praecox的转录组富含多样化和多态的微卫星.
已开发的EST-SSR标记物对于识别C. praecox品种具有特异性和有效性.
这些分子标记物有助于评估遗传多样性,并识别与C. praecox.重要的特征相关的基因.

关键词:

美国东部 - 苏联苏联.在RNA-seqqq.集群化是一种集群化.标识标识标识标识标识多形态主义的多态主义.

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相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...