相关实验视频

Updated: Jun 17, 2025

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Published on: August 1, 2018

KLHL14和E-Cadherin核共同表达作为非功能性PitNET侵入性的预测因素:初步研究

Jacopo Berardinelli¹, Valentina Russo², Angelo Canciello²

¹Department of Neurosciences, Reproductive and Odontostomatological Sciences, Division of Neurosurgery, University of Naples "Federico II", 80138 Naples, Italy.

Journal of clinical medicine

|August 10, 2024

概括

核表达E-cadherin和KLHL14蛋白与非功能性垂体神经内分泌瘤的侵入性相关. 较低的KLHL14水平可能表明更好的无进展生存率,表明预后潜力.

科学领域:

内分泌学在内分泌学.
在瘤学瘤学.
分子生物学分子生物学

背景情况:

非功能性垂体神经内分泌瘤 (NF-PitNETs) 需要改进诊断和治疗策略.
表皮-介质细胞过渡 (EMT) 涉及到NF-PitNET的致病性.
E-cadherin (E-Cad) 和KLHL14是EMT中的关键蛋白质,并作为预后标记物进行了研究.

研究的目的:

分析E-cadherin和KLHL14作为NF-PitNET中的预后标记物的表达.
调查E-Cad和KLHL14在瘤侵入性方面的亚细胞局部.
探索蛋白质表达,局部化和临床结果之间的相关性.

主要方法:

免疫组织化学被用来描述KLHL14和E-Cad亚细胞表达在12个NF-PitNET手术标本.
患者被分为低 (Ki67 < 3%) 和高 (Ki67 ≥ 3%) 的侵入性组.
在健康的,低侵入性和高侵入性NF-PitNET组织中分析了蛋白质表达和局部化.

主要成果:

与健康组织相比,NF-PitNET组织中KLHL14表达和核转位增加.
无论是E-Cad还是KLHL14,都表现出从细胞质转向核定位的转变,瘤的侵入性增加.
在E-Cad/KLHL14同定位 (细胞质和核) 和NF-PitNET侵入性等级 (p=0.01) 之间观察到显著的相关性.

结论:

在高级侵入性NF-PitNET中,E-Cad和KLHL14的核积累表明,驱动局部瘤入侵的新奇机制.
在KLHL14表达较低的患者中,观察到延长无进展生存的趋势.
需要对更大的队列进行进一步的研究,以确认KLHL14在NF-PitNETs中的预后意义.

关键词:

这是E-cadherin.在KLHL14HL14上表皮介质介质的过渡.不起作用的垂体腺瘤.下垂体腺瘤的前列腺瘤.

更多相关视频

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Three-Dimensional 3D Tumor Spheroid Invasion Assay

Published on: May 1, 2015

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

A Model for Perineural Invasion in Head and Neck Squamous Cell Carcinoma

Published on: January 5, 2017

相关概念视频

Cadherins in Tissue Organization

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...

Structure of Cadherins

Structure of Cadherins

The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...