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用酸盐和酸盐基分离盐制备的加工奶酪中的可溶盐
Gaurav Kr Deshwal1,2, Laura G Gómez-Mascaraque1, Mark Fenelon1
1Department of Food Chemistry and Technology, Teagasc Food Research Centre, P61 C996 Fermoy, Ireland.
Molecules (Basel, Switzerland)
|August 10, 2024
概括
这项研究研究了加工奶酪中的分离盐 (CSS),发现三酸盐 (TSC) 允许更多的通过过器,而不是二聚酸盐 (DSPP) 或六酸 (SHMP). 水溶性提取物方法有效地分析了加工奶酪中的盐分布.
科学领域:
- 食品科学 食品科学 食品科学
- 乳制品化学 乳制品化学
- 蛋白质化学 蛋白质化学
背景情况:
- 加工奶酪 (PC) 的质量受蛋白质和盐分发的影响.
- 分离盐 (CSS) 在加工奶酪的功能中起着至关重要的作用.
- 了解盐蛋白相互作用是优化加工奶酪质地和稳定性的关键.
研究的目的:
- 为了确定加工奶酪中的蛋白质和矿物盐 (Ca,P,Na,Mg) 的分布.
- 评估不同分离盐 (二酸盐,二酸盐,六酸,三酸) 对盐和蛋白质溶解性的影响.
- 评估水溶性提取物 (WSE) 方法用于分析加工奶酪中的盐分布的有效性.
主要方法:
- 制备加工奶酪样品,含有不同水平 (180或360mEq/kg) 的CSS.
- 从加工奶酪中提取一种水溶性分量 (WSE).
- 超离心 WSE 分离可溶性和颗粒物组成部分.
- 对盐 (Ca,P,Na,Mg) 和蛋白质含量的不同分数的分析,包括10kDa的透物.
主要成果:
- 经过加工的奶酪样本显示水分为45-46%,脂肪为26-27%,蛋白质为20-21%,pH值为5.2或5.7.
- 超离心法表明,WSE中的大多数蛋白质是不可沉积的.
- 在pH值为5.7的加工奶酪中观察到更高的WSE蛋白质含量,而在相同的CSS度下,pH值为5.2.
- 对于二氧化酸盐 (DSPP) 和六甲酸盐 (SHMP),WSE中55-85%的Ca和P是10kDa的透性,这表明不可透的Ca-多酸盐-素复合物.
- 对于三酸盐 (TSC),WSE中超过90%的Ca是10kDa的透性,这表明细胞通过结细胞的破坏.
结论:
- 水溶性提取物 (WSE) 方法适用于研究加工奶酪中的盐分布.
- 10kDa WSE超离心超的透物提供了最准确的可溶盐的评估,不包括与蛋白质相关的盐.
- 不同的CSS表现出不同的机制,影响盐和蛋白质溶解度以及加工奶酪中的复杂形成.
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