用于模仿胃肠道蛋白质消化的实验协议:系统性审查
Anna Beatriz Santana Luz1,2, Amanda Fernandes de Medeiros1, Gidyenne Christine Bandeira Silva de Medeiros3,4
1Biochemistry and Molecular Biology Postgraduate Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal 59064-741, RN, Brazil.
Nutrients
|August 10, 2024
概括
本综述确定了20项试验室研究,模拟了人类蛋白质消化过程. INFOGEST模型被认为是研究生物活性和代谢途径最有效的模型.
科学领域:
- 生物化学 生物化学
- 生理学 生理学 生理学
- 食品科学 食品科学 食品科学
背景情况:
- 来自原生蛋白质的生物活性会影响新陈代谢途径.
- 模拟人类蛋白质消化对于理解这些影响至关重要.
- 现有的文献缺乏使用适当实验模型的研究的综合观点.
研究的目的:
- 系统地审查和收集模拟人类蛋白质消化的实验模型的证据.
- 确定研究蛋白质消化过程中最有效的模型.
- 分析在体外蛋白质消化研究中使用的方法和条件.
主要方法:
- 在多个数据库 (PubMed,Web of Science,ScienceDirect,Embase,虚拟健康图书馆,Scopus) 进行系统的文献搜索.
- 纳入标准侧重于模拟人类蛋白质消化的体外研究.
- 使用卫生评估和翻译指南办公室评估的方法质量.
- 分析蛋白质来源,消化阶段,酶使用,pH条件和采用的模型.
主要成果:
- 从1985年初始文章中确定了20项符合条件的体外研究.
- 研究了基于植物和动物的蛋白质来源.
- 在65%的研究中采用了INFOGEST统一静态模型,并被认为是非常有效的.
- 常见的消化持续时间为120分钟,在特定的pH水平下经常使用粉酶,素和胰腺素.
结论:
- 该INFOGEST模型提供了一个强大的和生理上相关的方法来模拟人类胃肠道蛋白质过程.
- 这一系统性审查强调了像INFOGEST这样的标准化模型对于未来对生物活性和代谢健康的研究的重要性.
- 利用这些模型进行进一步的研究可以解决有关蛋白质消化及其生理影响的关键问题.
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