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Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...

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Updated: Jun 17, 2025

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

Published on: February 18, 2009

比较转录组学用于识别微藻中的RNA编写器和删除器.

Luca Ambrosino¹, Alessia Riccardi², Melina S Welling³

¹Research Infrastructure for Marine Biological Resources Department, Stazione Zoologica Anton Dohrn, Via Acton 55, 80133 Napoli, Italy.

International journal of molecular sciences

|August 10, 2024

概括

这项研究在微藻中确定了RNA甲基转移酶 (写字体) 和脱甲基酶 (消光剂),揭示了它们在营养应激下的差异表达. 一些写作基因不在转录组中,但存在于基因组中,表明特定条件的表达.

关键词:

标志性转录组学标志性转录组学擦拭器擦拭器擦拭器微藻是一种微藻.压力反应应激反应作家作家写作的人.

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

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Last Updated: Jun 17, 2025

Analyzing Gene Expression from Marine Microbial Communities using Environmental Transcriptomics

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Published on: February 18, 2009

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Use of Alu Element Containing Minigenes to Analyze Circular RNAs

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科学领域:

史诗转录组学史诗转录组学
分子生物学分子生物学
海洋微生物学海洋微生物学

背景情况:

经转录组学代表了真核生物中的新型调节层,对发育和应激适应至关重要.
RNA甲基转移酶 (编写者) 和脱甲基酶 (删除者) 是表体转录学调节的关键参与者.

研究的目的:

在四种微藻中识别RNA编写和擦除酶: *Alexandrium tamutum*, *Amphidinium carterae*, *Cylindrotheca closterium*和 *Tetraselmis suecica*.
为了研究这些酶在营养饥饿压力下的表达模式.

主要方法:

用已知的植物序列作为查询来生物信息识别RNA编写和擦除基因.
全基因组序列相似性搜索以确认基因存在.
在各种营养应激条件下 (耗,耗,添加) 在A. carterae*中进行基因表达分析.

主要成果:

大多数已知的植物RNA编写和除基因在研究的微藻中被确定,除了MTA,VIRILIZER和HAKAI.
序列相似性搜索证实了MTA,VIRILIZER和HAKAI在基因组中的存在,这表明它们在转录组中的缺席是由于缺乏表达.
在营养应激条件下观察到已识别的基因的差异表达,特别是在 *A. carterae*.

结论:

微藻类拥有一套保存的RNA表转录机械,类似于植物.
特定RNA编写和除基因的表达受营养物质的可用性和其他环境压力因素的调节.
这项研究提供了关于表体转录学在微藻应激反应和基因调节中的作用的基础见解.