由Bacillus licheniformis生产的外聚合物的抗氧化和乳化活性
Sánchez-León Enrique1, Amils Ricardo1,2, Abrusci Concepción1,2
1Departamento de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, UAM, Cantoblanco, 28049 Madrid, Spain.
International journal of molecular sciences
|August 10, 2024
概括
这项研究引入了一种来自Bacillus licheniformis的新型外聚糖 (EPS),具有强大的抗氧化和乳化特性. 非有毒的EPS表现出显著的自由基清除和保护作用,使其适合于各种应用.
科学领域:
- 生物化学和生物技术
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 外聚糖 (EPS) 是微生物聚合物,具有多种应用.
- 细菌状菌产生具有独特结构和功能性质的外聚合物.
- 对生物相容和有效的抗氧化剂和乳化剂的需求正在增长.
研究的目的:
- 来自Bacillus licheniformis的外聚糖 (ESPp) 的特征 IDN-EC.
- 评估ESPp.的抗氧化,细胞毒性和乳化活性.
- 探索ESPp在环境和健康领域的潜在应用.
主要方法:
- ESPp被分离并以其组成和分子量为特征.
- 在HeLa细胞上使用MTT试验评估了细胞毒性.
- 通过DPPH,基和超氧化离子清理试验来确定抗氧化活性.
- 测量了酶活性 (CAT,GSH-Px) 和对H2O2的保护作用.
- 评估了各种植物油的乳化活性,并与商业表面活性剂进行了比较.
主要成果:
- 具有5000大分子重量的ESPp对HeLa细胞没有细胞毒性.
- 它表现出显著的自由基清除活动 (DPPH:65%,OH:98.7%,O2-:97%).
- ESPp增强了catalase和谷氨过氧化酶的活性,并保护细胞免受H2O2诱导的损伤.
- 外聚糖体对芝麻油 (72%) 和橄油 (50%) 具有有效的乳化特性,其性能优于一些商业表面活性剂.
结论:
- ESPp是一种无毒的外聚糖,具有强大的抗氧化剂和自由基清除能力.
- 它的强烈乳化活性,特别是芝麻油,表明在食品或化品行业的潜力.
- 抗氧化,无毒和乳化性质的结合使ESPp成为环境修复和与健康有关的应用的有希望的候选者.
相关概念视频
01:20Radical Autoxidation
The oxidation of an organic compound in the presence of air or oxygen is called autoxidation. For example, cumene reacts with oxygen to form hydroperoxide. Autoxidation involves initiation, propagation, and termination steps. Many organic compounds are susceptible to autoxidation—especially ethers in the presence of oxygen, which form hydroperoxides. Even though this reaction is slow, old ether bottles contain small amounts of peroxide, which leads to laboratory explosions during ether...
01:20Endospores and Sporulation
Endospores are specialized, dormant cells primarily formed by Gram-positive bacteria, including Bacillus and Clostridium, enabling survival under extreme environmental conditions. Due to their unique composition and formation process, these structures are highly resistant to physical and chemical insults, such as extreme heat, ultraviolet and ionizing radiation, desiccation, and toxic chemicals. Rare instances of endospore-like structures have also been observed in some Gram-negative bacteria,...
01:25Production of Organic Acids
Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...