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对瓶类型根茎根的转录组分析确定了对低根区温度压力反应的新型转录因子

Jinqiu Liu^1,2, Man Zhang^1,2, Jian Xu^1,2

¹Institute of Vegetable Crop, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

International journal of molecular sciences

|August 10, 2024

概括

瓶根在低根区温度 (LRT) 下显示氧化应激和抗氧化酶活性增加. 这项研究确定了参与LRT反应的关键基因和转录因子,有助于开发耐寒的根茎.

科学领域:

植物科学植物科学
分子生物学分子生物学
农业科学农业科学

背景情况:

瓶 (Lagenaria siceraria) 是西瓜的重要根茎,但低温阻碍了它的生长和营养吸收.
了解瓶对低根区温度 (LRT) 的根反应对于改善根茎的寒冷耐受性至关重要.

研究的目的:

研究瓶根对LRT压力的反应的生理和分子机制.
为了确定涉及LRT耐受性的差异表达基因 (DEGs) 和转录因子 (TFs).

主要方法:

在控制和LRT条件下对瓶根的生理特征和转录基因特征进行比较分析.
通过RNA测序 (RNA-Seq) 在LRT暴露48h和72h后识别DEGs.
定量实时聚合酶链反应 (qRT-PCR) 用于验证DEG表达模式.

主要成果:

慢性激素治疗增加了氧化应激标志物 (MDA,ROS) 和增强了抗氧化酶活性 (SOD,POD).
RNA-Seq分别在48小时和72小时确定了2507个和1326个DEG,包括174个和271个TF.
通过qRT-PCR确认了WRKY,NAC,bHLH,AP2/ERF和MYB TFs的表达模式.

结论:

这项研究为了解瓶根中的LRT响应TFs提供了基础.
识别的基因和TFs可以通过分子育种来促进瓶根茎的寒冷耐受性.

关键词:

在RNA-Seqq.一个瓶子的子较低的根区温度.根源根源根源根源转录因子的转录因子

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