在接受干细胞教育疗法后,在1型糖尿病患者的外周血液胰岛素产生细胞 (PB-IPC) 上,葡萄糖载体2 (GLUT2) 的表达增加
Yong Zhao1, Boris Veysman1, Kristine Antolijao2
1Throne Biotechnologies, Paramus, NJ 07652, USA.
International journal of molecular sciences
|August 10, 2024
概括
干细胞教育疗法可增强1型糖尿病 (T1D) 患者周围血液胰岛素生成细胞 (PB-IPC) 上的葡萄糖转运体2 (GLUT2) 表达. 这种治疗改善了小岛细胞功能和临床结果,为其他自身免疫性疾病提供了潜力.
科学领域:
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
- 再生医学是一种再生医学.
背景情况:
- 1型糖尿病 (T1D) 是一种自身免疫性疾病,其特征是破坏产生胰岛素的β细胞.
- 周围血液胰岛素生成细胞 (PB-IPC) 表达葡萄糖转运体2 (GLUT2),对于感知葡萄糖至关重要.
- 以前的研究表明,在自身免疫性疾病中,干细胞教育疗法的潜力很大.
研究的目的:
- 在T1D患者中研究PB-IPC上的GLUT2表达.
- 评估干细胞教育治疗对GLUT2表达和T1D中的免疫标记物的影响.
- 探索干细胞教育治疗对其他自身免疫和炎症相关疾病的治疗潜力.
主要方法:
- 利用食品和药物管理局 (FDA) 批准的2期临床研究,涉及干细胞教育疗法.
- 隔离的外周血液单核细胞 (PBMC) 用于流细胞计分析.
- 在治疗前和治疗后评估PB-IPC,GLUT2表达和炎症性细胞因特鲁金-1β (IL-1β) 水平.
主要成果:
- T1D患者在PB-IPC上表现出低基线GLUT2表达.
- 在T1D患者中,干细胞教育疗法显著上调了PB-IPC上的GLUT2表达.
- 治疗降低了IL-1β水平,增加了GLUT2+CD45RO+PB-IPC百分比,类似于其他自身免疫疾病患者.
结论:
- 干细胞教育疗法通过调高T1D患者的GLUT2表达来恢复PB-IPC功能.
- 该疗法改善了T1D的临床结果,并显示出治疗其他自身免疫和炎症相关疾病的前景.
- 这些发现阐明了干细胞教育疗法的分子机制,扩大了其治疗适用性.
相关概念视频
Diabetes Mellitus: Type 2 and Gestational
2.3K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
2.3K
Glucose Homeostasis: Pancreatic Islets and Insulin Secretion
1.2K
The pancreatic islets comprising only 1%-2% of the volume are highly vascularized and innervated mini-organs. They contain five endocrine cell types, including β cells that secrete insulin, which is synthesized as a single polypeptide chain, preproinsulin, processed to proinsulin, and finally to insulin and C-peptide. This process is complex and regulated, involving the Golgi complex, the endoplasmic reticulum, and the secretory granules of the β cell.
Insulin and C-peptide are...
Insulin and C-peptide are...
1.2K
Carbohydrate Metabolism
10.9K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
10.9K
Hormones Regulating Blood Glucose
3.2K
Insulin is released by beta cells of the pancreas when blood glucose levels are high. It facilitates glucose absorption and utilization in insulin-dependent cells with insulin receptors on their plasma membranes. Insulin promotes glucose uptake by increasing the number of glucose transport proteins in the cell membrane, allowing glucose to enter the cell. As a result, glucose utilization and ATP production are enhanced.
In addition to accelerating glucose uptake and utilization, insulin has...
In addition to accelerating glucose uptake and utilization, insulin has...
3.2K
Insulin Secretory Vesicles
4.9K
Insulin secretory vesicles release insulin to stimulate blood glucose uptake and regulate carbohydrate metabolism. When the blood glucose levels increase, glucose enters the pancreatic β-islet cells through glucose transporters. Once inside, glucose is metabolized through glycolysis, the citric acid cycle, and the electron transport chain, producing ATP. This increase in ATP concentration closes ATP-sensitive potassium channels, leading to depolarization of the membrane and the opening of...
4.9K
Diabetes Mellitus: Overview and Type I Subtype
2.5K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
2.5K


