普兰塔吉尼斯精液改善高尿血症由氧酸盐诱导
Tian Liu1, Liting Wang1, Li Ji1
1Key Laboratory of TCM-Information Engineer of State Administration of TCM, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China.
International journal of molecular sciences
|August 10, 2024
概括
普兰塔吉尼斯精液 (PS) 通过逆转损伤和调节尿酸水平,有效治疗高尿血症 (HUA). 这项研究确定了PS组件,并揭示了其机制涉及PPAR信号通路.
科学领域:
- 药理学和中国传统医学
- 代谢障碍 研究 研究 代谢障碍
- 腎臟病學 (nephrology) 是一種醫學專業.
背景情况:
- 植物精液 (PS) 是一种传统的中医药,用于治疗高尿血 (HUA) 和慢性病.
- 针对HUA的PS的特定化学成分和治疗机制在很大程度上仍未被阐明.
- 了解这些方面对于验证其治疗潜力和优化其应用至关重要.
研究的目的:
- 为了确定 Plantaginis 种子 (PS) 的主要化学成分.
- 阐明PS在治疗高尿血症 (HUA) 中的潜在机制.
- 探索PS对与HUA相关的基因表达特征的影响.
主要方法:
- 超高性能液态染色学与四极轨道轨道质谱学 (UPLC-QE-Orbitrap-MS) 结合用于组件识别.
- RNA测序 (RNA-seq) 用于分析对PS治疗反应的基因表达变化.
- 实时定量PCR (RT-qPCR) 和西式涂抹用于基因和蛋白质表达的验证.
主要成果:
- 在PS中确定了39个成分,其中13个在治疗后的老鼠血清中检测到.
- PS治疗逆转了损伤,降低了血清尿酸 (UA),山丁氧化酶 (XOD) 和细胞因子水平.
- PS调节脏尿酸载体1 (URAT1) 和葡萄糖载体9 (GLUT9) 的水平,并显著改变了PPAR,甘氨酸/氨酸/氨酸和脂肪酸代谢途径.
结论:
- 这项研究全面描述了化学成分,并阐明了Plantaginis精液 (PS) 治疗高尿血症 (HUA) 的治疗机制.
- 通过调节UA运输和代谢通路,特别是PPAR信号通路,PS显示了治疗效果.
- 植物的精液显示出作为中国传统医药用于预防和治疗HUA的前景.
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