水作为可持续样品制备的绿色溶剂:从洛萨坦片中单滴微提取N-尼托拉胺
Natalia Gabrielly Pereira Dos Santos1, Deyber Arley Vargas Medina1, Fernando Mauro Lanças2
1University of São Paulo, São Carlos Institute of Chemistry, São Carlos, SP, Brazil.
Analytical and bioanalytical chemistry
|August 10, 2024
概括
这项研究引入了水作为一个环保溶剂,用于头部单滴微提取 (HS-SDME),以检测洛萨坦片中的N-尼托拉胺. 绿色方法简化了使用HPLC-UV-Vis的分析,为制药污染物测试提供了一个可持续的替代方案.
科学领域:
- 分析化学 分析化学
- 绿色化学 绿色化学
- 药品分析 药品分析
背景情况:
- 水是一种可持续且无毒的溶剂,在微观提取方面仍有未开发的潜力.
- 药品中N-尼托拉胺分析的传统方法经常面临矩阵效应,需要复杂的检测技术.
- 微型样品制备技术对于高效和环保的化学分析至关重要.
研究的目的:
- 率先使用水作为提取溶剂在头部空间单滴微提取 (HS-SDME) 的N-尼托胺.
- 开发一种自主,绿色的微提取方法,用于制药分析.
- 使用HPLC-UV-Vis. 在洛萨坦片中直接检测N-尼托拉胺.
主要方法:
- 自主HS-SDME使用Arduino控制的实验室制造的卡特西亚机器人进行.
- 用水作为提取溶剂在线预缩和直接注入柱交换系统.
- 为了优化关键的实验参数,进行了单变量和多变量实验.
主要成果:
- 基于水的HS-SDME方法有效地消除了制药成分和辅助剂的矩阵效应.
- 分析简化为具有紫外线检测 (HPLC-UV-Vis) 的高性能液态色谱.
- 该方法证明了线性反应 (1002400 ng g-1),具有良好的检测性,精度和准确性.
结论:
- 开发的方法提供了一种高效和绿色的替代方法,用于确定制药产品中危险的N-尼托胺.
- 在HS-SDME中使用水作为提取溶剂显著减少了对环境的影响.
- AGREEprep方法证实了这种新的分析方法的卓越环境可持续性.
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