孟买血型 (Oh) 印度裔个体中的ACKR1基因多态
Roshan Shaikh1, Ghosh Kanjaksha2, Vasantha Kashivishwanath1
1Department of Transfusion Medicine, India.
概括
这项研究调查了印度罕见的孟买 (OH) 血型表型的个体的达菲血型系统 (ACKR1) 基因多态性. 结果揭示了O个人的特定FY等位基因频率和单位基因分布,包括首次证明某些弱抗原基因型.
科学领域:
- 遗传学 是一个遗传学.
- 免疫学 免疫学 免疫学
- 血液学 血液学 血液学
背景情况:
- 达菲血型系统 (ACKR1) 具有显著的遗传多态性,具有多样化的人口分布.
- 了解这些多态性对于输血医学和人口遗传学至关重要.
研究的目的:
- 为了对ACKR1抗原进行基因定型,并确定Bombay (Oh) 血型表型的个体的FY等位基因频率.
- 为了研究ACKR1基因多态性在一个印度队列的OH血型.
主要方法:
- 来自印度孟买的160名Oh个体和100名非Oh献血者的ACKR1表型和基因型频率的基因定型.
- 使用聚合酶链反应-限制片段长度多态化 (PCR-RFLP) 进行了分子基因定型.
主要成果:
- 在这两组中,T等位基因和TT基因型在T-67) C多态性中占主导地位.
- 没有ACKR1无 (Fya-b-) 现型. FYA/FYB,FYA/FYA和FYB/FYB基因型在OH和非OH组之间显示出不同的频率.
- 在Oh和非Oh个体之间观察到TGGGC和TGGGT亚型频率的显著差异.
结论:
- 包括弱抗原在内的ACKR1基因多态性在OH个体中普遍存在,TGGGC单体的高频率.
- 这项研究提供了FyBweak,FyAweak和FyAweak/FyBES基因型在印度受试者红细胞膜上的FyBweak,FyAweak和FyAweak/FyBES基因型的第一个证据.
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