相关实验视频

Updated: Dec 30, 2025

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Preparation, Administration, and Assessment of In Vivo Tissue-Specific Cellular Uptake of Fluorescent Dye-Labeled Liposomes

Published on: July 30, 2020

优化抗生素加载的PLGA纳米颗粒的细胞内传递到巨细胞

Alhareth A Alsa'd¹, Michelle K Greene², May Tayyem³

¹School of Pharmacy, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK; Pharmacological and Diagnostic Research Centre, Faculty of Pharmacy, Al-Ahliyya Amman University, Amman, Jordan.

International journal of pharmaceutics

|August 10, 2024

概括

较大的多样乳糖合糖酸 (PLGA) 纳米颗粒可以改善对巨细胞的抗生素输送,增强细胞内K. pneumoniae感染的治疗. 然而,疗效因细菌及其在宿主细胞内的位置而异.

科学领域:

生物医学工程生物医学工程
纳米技术纳米技术
传染性疾病传染性疾病

背景情况:

细菌可以通过居住在宿主细胞中来逃避抗生素,就像巨细胞一样.
开发有效的细胞内抗菌素输送系统对于治疗持久性感染至关重要.

研究的目的:

调查聚乳糖糖酸 (PLGA) 纳米颗粒大小对抗生素输送和对细胞内细菌的疗效的影响.
确定最佳的纳米粒子特性,以提高巨细胞的吸收.

主要方法:

合成不同大小的罗达胺-B结合的PLGA纳米粒子.
在巨细胞和上皮细胞系中纳米粒子吸收的评估.
在共同培养模型中评估抗生素加载的PLGA纳米颗粒对细胞内K. pneumoniae和S. aureus的疗效.

主要成果:

巨细胞对PLGA纳米颗粒的吸收随着直径越大而增加.
增加纳米颗粒大小显著提高了对细胞内K. pneumoniae的疗效.
对细胞内黄金色杆菌的有效性没有得到改善,可能是由于纳米颗粒无法进入细菌区的原因.

结论:

优化PLGA纳米颗粒大小可以增强针对特定细胞内细菌感染的抗菌疗效,例如K. pneumoniae.
取决于尺寸的纳米粒子输送的有效性是病原体特异的,取决于细胞内定位.

关键词:

抗生素抗生素是一种抗生素.感染感染感染细胞内细胞内细胞内.巨细胞是一个巨细胞.纳米颗粒是如何形成的在 PLGA PLGA 中.

更多相关视频

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Polyethyleneimine-coated Iron Oxide Nanoparticles as a Vehicle for the Delivery of Small Interfering RNA to Macrophages In Vitro and In Vivo

Published on: February 5, 2019

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

相关概念视频

Bioavailability Enhancement: Drug Permeability Enhancement

Bioavailability Enhancement: Drug Permeability Enhancement

Body:After oral administration, poor permeability often limits the rate at which drugs are absorbed through the intestinal epithelium. Enhancing drug permeability is crucial for effective therapy, and several strategies have been developed to overcome this challenge.One effective strategy involves the use of lipid-based formulations. These formulations enhance dissolution and solubility, targeting physiological mechanisms to increase drug absorption. This includes stimulating bile salt...