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角膜上皮质修复,亡和免疫调节中的介质细胞与表皮细胞外囊:一个体外研究

Vasudeva Tati¹, Sai Muthukumar V², Sachin Shukla¹

¹Prof. Brien Holden Eye Research Centre, Hyderabad Eye Research Foundation, L V Prasad Eye Institute, Hyderabad, 500034, India; Sudhakar and Sreekanth Ravi Stem Cell Biology Laboratory, Centre for Ocular Regeneration, L V Prasad Eye Institute, Hyderabad, 500034, India.

Experimental eye research

|August 10, 2024

概括

人类骨髓介质干细胞衍生的细胞外囊泡 (BM-MSC-EVs) 加快角膜上皮的愈合,减少细胞亡,并改善抗炎性质,相比于角膜上皮细胞衍生的细胞外囊泡在体外.

科学领域:

眼科医生眼科眼科
再生医学是一种再生医学.
细胞生物学细胞生物学

背景情况:

角膜损伤会导致上皮损伤,影响视力.
介质细胞干/干细胞 (MSCs) 衍生的细胞外囊泡 (EVs) 提供无细胞治疗潜力.
源自人类角膜上皮细胞的内源性EVs (HCEC-EVs) 需要对角膜修复进行调查.

研究的目的:

从HCE-2细胞 (HCEC-EVs) 中分离和描述EVs.
评估HCEC-EVs在角膜上皮质修复,抗亡和免疫调节方面的潜力.
在体外比较HCEC-EVs与人类骨髓介质干细胞衍生的EVs (BM-MSC-EVs).

主要方法:

使用纳米粒子跟踪分析和西式涂抹进行EV隔离和表征.
在体外伤口愈合测定用于角膜上皮质修复.
流细胞测量用于细胞亡和ELISA用于细胞因子表达 (IL-6,TNF-α,IL-10,TGF-β).

主要成果:

无论是BM-MSC-EV还是HCEC-EV,都显示出类似的形态 (<150 nm) 与特定的外体标记 (CD63,CD81,TSG101).
在HCEC中,EV的数量高于BM-MSC.
与HCEC-EVs相比,BM-MSC-EVs在体外显示出优异的角膜上皮愈合,抗亡作用和抗炎性质.

结论:

BM-MSC-EVs在角膜上皮质修复方面显示出比HCEC-EVs在体外更大的治疗潜力.
来自这两种来源的EV具有潜在的治疗应用的关键特征.
需要进一步的体内研究来验证这些发现的临床转化.

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