相关实验视频

Updated: Jun 17, 2025

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Monitoring Intraspecies Competition in a Bacterial Cell Population by Cocultivation of Fluorescently Labelled Strains

Published on: January 18, 2014

在Cupriavidus necator H16中HCO₃-吸收和CO₂固定的功能性可塑性

Justin Panich¹, Emili Toppari¹, Sara Tejedor-Sanz²

¹Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.

Bioresource technology

|August 10, 2024

概括

通过修改其无机碳代谢,工程Cupriavidus necator H16的碳固定是可能的. 用多种溶解的无机碳转运体取代本地系统,恢复了自营生长并增强了细胞密度.

科学领域:

微生物学微生物学
合成生物学合成生物学
生物化学生物化学

背景情况:

库普里亚维杜斯 (Cupriavidus necator H16) 是一个突出的微生物,其工程无机碳吸收和固定的潜力尚未得到充分探索.
了解其无机碳代谢对于推进基于二氧化碳的生物制造至关重要.

研究的目的:

研究内源性和异源性基因在Cupriavidus necator H16无机碳代谢中的作用.
探索工程策略,以提高C. necator.中的无机碳吸收和固定.

主要方法:

基因操纵Cupriavidus necator H16,包括基因删除和替代.
测试改变碳代谢对自营生长和细胞密度的影响.
评估异构溶解无机碳 (DIC) 载体和鲁比斯科同类物质的功能.

主要成果:

删除β-碳酸无水酶显著损害了自营生长.
用异质DIC载体取代原生系统恢复了自营生长和增加了细胞密度.
表达Halothiobacillus neopolitanus DAB2和各种rubisco同类的工程菌株显示出与野生类型相似的生长.

结论:

碳酸脱酶主要支持在C. necator的自营生长期间的阿纳皮洛斯代谢.
多种DIC输送器可以功能地补充本地碳吸收系统.
这项研究强调了C. necator对HCO3吸收和CO2固定的代谢灵活性,为基于CO2的生物制造开辟了道路.

关键词:

生物制造生物制造生物制造 C1代谢过程中的代谢转换过程中的二氧化碳 (CO2) 转换.克雷格 (Crage) 的意思碳酸无水酶是一种碳酸无水酶.DAB2 DAB2 DAB2 是一个非常重要的数据库.气体发酵发酵过程中的气体.基因组工程是基因组工程.鲁比斯科 (Rubisco) 是一个古老的葡萄酒.

更多相关视频

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic Rate

Published on: December 12, 2015

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

A Toolkit to Enable Hydrocarbon Conversion in Aqueous Environments

Published on: October 2, 2012

相关概念视频

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...

Overview of Metabolism

Overview of Metabolism

Living cells constantly carry out various chemical reactions which are necessary for their proper functioning. These reactions are interlinked to one another via multiple pathways. The collection of these chemical reactions is known as metabolism.
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

Bioremediation

Bioremediation

Bioremediation is the use of prokaryotes, fungi, or plants to remove pollutants from the environment. This process has been used to remove harmful toxins in groundwater as a byproduct of agricultural run-off and also to clean up oil spills.