体对体基因组组合的Eleocharis dulcis和表达形状在体发育过程中
Yang Chen1,2, Xinyi Zhang2, Lingyun Wang1
1Provincial Key Laboratory of Characteristic Aquatic Vegetable Breeding and Cultivation, Jinhua Academy of Agricultural Sciences (Zhejiang Institute of Agricultural Machinery), Jinhua, Zhejiang, 321000, China.
Scientific data
|August 10, 2024
概括
研究人员组装了完整的中国水栗 (Eleocharis dulcis) 基因组,揭示了其进化和基因表达的洞察力. 这为了解其种植和营养价值提供了基础.
科学领域:
- 植物基因组学 植物基因组学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- (Eleocharis dulcis) 是一种全球种植的水生蔬菜,因其营养和味道而受到重视.
- 一个高质量的参考基因组对于理解它的遗传基础,进化和改进至关重要.
研究的目的:
- 为了组装一个端粒到端粒 (T2T) 基因组的Eleocharis dulcis.
- 研究基因组进化,包括全基因组复制 (WGD) 事件.
- 分析基因表达模式在培养与野生加入期间的角质发育.
主要方法:
- 整合牛津纳米孔 (ONT) 超长读数,PacBio长读数和Hi-C数据用于基因组装.
- 将基因组固定在111个无间隙染色体上.
- 比较的时间过程转录组分析的 corm 发展.
主要成果:
- 一个493.24 Mb的E. dulcisT2T基因组组合,包含48.31%的重复元素和33493个蛋白质编码基因.
- 证据表明,在从Rhynchospora breviuscula ~35.6 Mya分离后的进化过程中发生了多次全基因组重复 (WGD) 事件和染色体破裂.
- 在芽发育过程中鉴定了15870个差异表达基因 (DEGs),特别是在中部胀阶段,在粉代谢中进行丰富.
结论:
- T2T基因组为E. dulcis研究提供了高分辨率的资源.
- 基因组进化涉及显著的重复和重排事件.
- 不同的基因表达,特别是在粉代谢中,在玉米发育过程中,在栽培和野生中国水栗之间存在差异.
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