基于基因组表征和比较基因组分析的Morchella上的Pseudodiploöspora longispora的进化和相关的致病基因
Jiangtao Xie1,2, Xue Liu1,2, Zaili Qin1,2
1Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, Guizhou, China.
Scientific reports
|August 10, 2024
概括
确定了一种新的真菌,Pseudodiploöspora longispora,它在可食用的菌中引起白病. 它的基因组分析揭示了与其他病原体的共同特征以及毒素产生的潜在食品安全风险.
科学领域:
- 菌类学 菌类学是指菌类学.
- 植物病理学 植物病理学
- 基因组学就是基因组学.
背景情况:
- 真正的 (Morchella) 是一种有价值的食用和药用,面临着白病的重大威胁.
- 引起白病的真菌病原体表现出不同的宿主适应能力和破坏能力,需要进行详细的调查.
- 了解这些病原体对于开发 Morel 种植中有效的疾病管理战略至关重要.
研究的目的:
- 隔离,识别和表征主要的真菌病原体,负责来自贵州省的鱼白病.
- 执行全基因组测序和分析已识别的病原体,以了解其遗传构成和进化关系.
- 为了比较已识别的病原体与其他已知的Morel病原体关于酶能力和二次代谢物生产.
主要方法:
- 从受感染的中分离和净化致病剂.
- 形态,分子 (DNA测序) 和致病性测试用于病原体识别.
- 高通量基因组测序,组装,基因预测和家族遗传学分析.
- 对碳水化合物降解酶和二次代谢物基因集群的比较分析.
主要成果:
- 这种白色的菌病原体被确定为Pseudodiploöspora longispora.
- 组装了P. longisporus的高质量基因组,预测了7381个蛋白质编码基因.
- 遗传学分析显示P. longisporus与Zelopaecilomyces penicillatus的最亲密的关系,大约在5000万年前分离.
- P. longisporus,Z. penicillatus和Cladobotryum protrusum共享了丰富的细胞壁降解酶 (酸酶,葡萄酸酶).
- P. longisporus和Z. penicillatus具有类似的leucinostatins基因集群;P. longisporus和C. protrusum含有Ochratoxin A.的基因集群.
结论:
- 长 Pseudodiploöspora被确定为莫雷尔的重要病原体.
- 长杆菌的基因组提供了对其病原性的洞察力,特别是对宿主降解的酶机制.
- 在P. longisporus和C. protrusum中存在Ochratoxin A基因集群,这表明存在潜在的食品安全问题.
- 这项研究为了解和管理更多疾病提供了有价值的基因组数据.
相关概念视频
02:54Evolutionary Relationships through Genome Comparisons
5.7K
Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
5.7K
02:05Gene Evolution - Fast or Slow?
7.1K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
7.1K