在阿皮叶科中逐渐建立复杂的氨酸生物合成途径
Xin-Cheng Huang1, Huanying Tang2, Xuefen Wei1
1College of Horticulture, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China.
Nature communications
|August 10, 2024
概括
复杂氨酸 (CCs) 生物合成在阿皮叶科植物中通过基因重复和关键酶的新功能化而演变. 这种途径解释了CC的产生和物种间的结构多样性.
科学领域:
- 植物生物化学和分子进化
- 代谢学和自然产品生物合成
背景情况:
- 复杂氨酸 (CCs) 是阿皮亚科植物中具有医学价值的代谢物,可能充当防御化合物.
- CC生物合成途径的进化起源和多样化在很大程度上仍未得到阐明.
研究的目的:
- 为了重建阿皮亚系中CC生物合成途径的进化史.
- 确定驱动CC生物合成和结构多样性的关键酶和遗传机制.
主要方法:
- 从34种阿皮亚ceae物种的基因组和转录组的家族遗传学分析.
- 基因克隆,异质表达,以及关键酶的体外酶分析.
- 确定影响酶活性的关键氨基酸变异.
主要成果:
- 三种必需的酶 (C2'H,C-PT,环酶) 通过基因重复 (异胎和并联) 在阿皮亚科的不同进化阶段产生.
- 这些酶的新功能化建立了CC路径.
- C-prenyltransferases (C-PTs) 是CC结构多样性 (线性/角性) 的关键驱动因素,通过准不同的贝利费隆位置 (C-6/C-8).
- 在C-PTs中的Ala161/Thr161氨基酸变异显著改变了酶活性和产品形成.
结论:
- 这项研究阐明了阿皮亚系中CC生物合成途径的进化轨迹.
- 这些发现解释了在阿皮亚ceae 种类中CC 生产的差异分布.
- 揭示了CC结构多样性的基础机制,与C-PT进化和特定的氨基酸替代有关.
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