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卡路里限制和拉巴胺明显减轻老化相关的小鼠肌肉中的蛋白质酸化变化

Meric Ataman^1,2, Nitish Mittal³, Lionel Tintignac⁴

¹Biozentrum, University of Basel, Basel, Switzerland. meric.ataman@unibas.ch.

Communications biology

|August 10, 2024

概括

卡路里限制 (CR) 和拉巴胺素 (RM) 治疗影响小鼠骨肌肉的蛋白酶. 这两种干预措施都减轻了衰老的影响,但CR显示的肌肉变化比RM更大.

科学领域:

衰老的研究研究.
分子生物学分子生物学
骨肌肉生理学骨肌肉生理学

背景情况:

卡路里限制 (CR) 和拉巴胺素 (RM) 已知可以减缓衰老并促进健康.
CR和RM对转录基因组和蛋白质组有部分重叠的影响.
在信号通路中,CR和RM对蛋白质酸化的影响仍然是无与伦比的.

研究的目的:

为了比较CR和RM对老鼠骨肌肉的蛋白质组的长期影响.
识别新型酸盐并分析它们的组织特异性.
了解营养感应通路抑制对与衰老相关的肌肉变化的影响.

主要方法:

在成年和老年小鼠的单体肌,前肌,三肌和腹肌肌中的蛋白质组的分析.
小鼠从15个月开始接受控制,CR或RM饮食.
6960个矿的可重复检测和广泛分析,包括1415个新网站.

主要成果:

在CR和RM干预中,长期对蛋白质组的长期影响大致一致,但在数量上是不同的.
这两种干预措施都在不同程度上减轻了骨肌肉中与年龄相关的变化.
与RM相比,CR在其影响中表现出更大的肌肉间变化.
鉴定了1415种新的酸,扩大了小鼠蛋白质组目录.

结论:

长期的CR和RM干预对小鼠骨肌肉蛋白酶有显著,明显的影响.
这些发现提供了关于组织特异性影响和与衰老和营养感应通路相关的新型酸盐的见解.
这项研究扩大了对CR和RM如何调节衰老期间骨肌肉中细胞信号的理解.

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