约克犬在中国小,Eriocheir sinensis的化过程中负面调节了Crustin表达

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Yuting Xi¹, Jialin Li¹, Zihao Wu¹

¹College of Aquaculture and Life Science, Dalian Ocean University, Dalian, 11026, China.

Developmental and comparative immunology

|August 11, 2024

概括

中国小的约克 (Yki) 基因在化过程中起到免疫作用. 它是由20-hydroxyecdysone诱导的,并通过抑制NF-κB.抑制抗微生物基因来抑制.

科学领域:

甲动物生物学甲动物生物学
免疫学免疫学免疫学
分子信号通道的分子信号通道.

背景情况:

化是甲类动物的一个关键过程,由20-基乙 (20E) 调节.
河马信号通路,涉及约克 (Yki) 转录因子,对于细胞生长和免疫反应至关重要.
在甲动物变过程中免疫调节机制需要进一步研究.

研究的目的:

在Eriocheir sinensis中识别和描述Yki基因 (EsYki).
调查EsYki在化周期期间对抗微生物基因表达的调节作用.
阐明EsYki在变过程中的免疫反应中的功能背后的分子机制.

主要方法:

在Eriocheir sinensis中对EsYki的鉴定和mRNA表达分析.
激素 (20E) 和细菌 (Aeromonas hydrophila) 的刺激实验.
使用特定抑制剂 (CA3) 抑制EsYki,并评估下游基因表达和蛋白质酸化 (NF-κB).

主要成果:

埃斯基基mRNA的表达在产卵前阶段是最高的,并在20E和A. hydrophila刺激后增加.
抑制EsYki导致抗微生物基因 (EsCrus1和EsCrus2) 的表达增加以及NF-κB酸化的升高.
在20E注射后,EsYki酸化升级,这表明该途径已被激活.

结论:

EsYki是由20E诱导的,在变过程中在抗微生物基因 (EsCrus) 的表达中起抑制作用.
EsYki通过抑制NF-κB信号传递来发挥其抑制作用.
这项研究增强了对类变过程中的免疫调节的理解.

关键词:

克拉斯斯是一个很棒的人.这里是Eriocheir sinensis的原点.免疫反应的免疫反应.化化化约克里的约克.

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