相关实验视频

Updated: Jun 17, 2025

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Author Spotlight: Exploring the Mechanisms of MicroRNA Loading into Extracellular Vesicles in Cancer Progression

Published on: October 6, 2023

无细胞和细胞外囊泡微RNAs对于固体瘤具有临床效用

Yoshinori Hayashi¹, Janelle-Cheri Millen², Romela Irene Ramos¹

¹Department of Translational Molecular Medicine, Saint John's Cancer Institute at Providence Saint John's Health Center, Santa Monica, CA, USA.

Molecular oncology

|August 12, 2024

概括

无细胞微RNAs (cfmiRs) 显示为癌症诊断,预后和治疗监测的生物标志物具有前途. 本综述强调了最近的进展,并确定了需要进一步研究以临床应用的领域.

科学领域:

生物标志物生物标志物
分子瘤学分子瘤学
翻译癌症研究翻译癌症研究

背景情况:

循环分子生物标记物,包括无细胞DNA (cfDNA),循环瘤细胞 (CTC) 和无细胞微RNA (cfmiRs),越来越多地被用于临床应用.
尽管取得了进展,但许多生物标志物的适用性,特异性,敏感性和可重复性仍然存在挑战.
翻译性研究显著提高了对cfmiRs在各种癌症中的理解和潜在的临床实用性的理解.

研究的目的:

审查过去五年关于固体瘤中cfmiRs的翻译研究.
总结cfmiRs在癌症诊断,预后和治疗监测中的潜在临床应用.
确定需要进一步研究和验证cfmiR临床实用性的领域.

主要方法:

使用PubMed进行的文献搜索.
包括/排除标准重点关注瘤类型,患者样本大小和临床应用.
确定了对固体瘤中cfmiRs的136项研究,按瘤类型,器官部位,cfmiR计数,方法和生物流体分析分类.

主要成果:

对于固体瘤中cfmiRs存在大量的转化研究.
研究分析了各种方面,包括瘤起源,cfmiR数量,分析方法和生物流体类型.
该审查巩固了关于cfmiRs在诊断,预后和治疗反应监测方面的潜力的发现.

结论:

cfmiRs代表了固体瘤的一个有前途的生物标志物.
进一步的研究和验证对于克服现有的挑战和实现其全部临床潜力至关重要.
未来研究的关键领域包括方法的标准化和更广泛的临床验证研究.

关键词:

cfmiRs 的使用情况.诊断诊断诊断的诊断诊断诊断诊断的诊断细胞外囊泡中的细胞外囊泡.流体分子活检液体分子活检预后预测预测的预测.固体瘤是一种固体瘤.

更多相关视频

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

Overview of Exosomes

Overview of Exosomes

Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...