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Updated: Jun 17, 2025

Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration
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Use of the TetON System to Study Molecular Mechanisms of Zebrafish Regeneration

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一个非侵入性的可光激活的Split-Cre重组酶系统用于斑马鱼的基因组工程

Ramy Elsaid1, Aya Mikdache1, Patricia Diabangouaya1

  • 1Institut Curie, PSL Research University CNRS UMR 3215, INSERM U934, 26 Rue d'Ulm, 75248 Paris Cedex 05, France.

iScience
|August 12, 2024
PubMed
概括

研究人员开发了一种新型的可光诱导的转基因系统,用于精确的体内细胞跟踪,使用斑马鱼中的Cre/loxP重组. 这种非侵入性工具为研究细胞命运和迁移提供了增强的时空控制.

关键词:
细胞生物学 细胞生物学分子生物学分子生物学

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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
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