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芯片上的椎间盘^MF:通过整合机械负载和动态媒体流来实现鼠标磁盘培养的新模式

Wanqing Xie¹, Yuan Xing^1,2, Li Xiao¹

¹Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA 22908, USA.

Advanced materials technologies

|August 12, 2024

概括

一种新的磁盘芯片MF模型成功维持了21天的ex vivo磁盘培养,在优化的机械负荷和营养下保持磁盘健康,与静态培养不同.

科学领域:

生物医学工程生物医学工程
再生医学是一种再生医学.
组织工程是组织工程.

背景情况:

椎间盘退化是背部疼痛的主要原因之一.
目前的ex vivo模型缺乏微环境的复杂性,无法准确研究磁盘健康状况.
了解机械负荷和营养效应对于开发疗法至关重要.

研究的目的:

开发和验证磁盘芯片MF系统用于ex vivo椎间盘培养.
研究机械负荷和营养对磁盘健康的综合影响.
建立一个研究磁盘退化和测试疗法的平台.

主要方法:

设计了一种多层微流体芯片,配备基于计算机Arduino的控制系统和机械加载单元.
优化了使用低档力传感器 (LPFS) 和计算模拟的轴向力测量.
维持ex vivo小鼠盘培养21天,优化机械负荷 (0.02MPa在1Hz) 和流量 (1μL/分钟).

主要成果:

磁盘在芯片上的MF系统维持了磁盘结构完整性和细胞健康21天.
与静态培养相比,在芯片上培养的磁盘显示了保存的原蛋白,降低了分解,并控制了酶活性.
在芯片模型中,磁盘细胞和原蛋白对齐有利于原生磁盘健康.

结论:

磁盘上芯片MF系统准确地模仿了体内磁盘微环境.
这个平台对于研究影响磁盘健康和退化因素非常有价值.
它为新型磁盘疗法的临床前测试提供了一个有前途的工具.

关键词:

生物反应器是一个生物反应器.椎间盘退化椎间盘退化机械负荷是指机械负荷.微流体中的微流体.器官在芯片上的器官

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