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在小鼠的内皮细胞中O-GlcNAc的修饰通过从肠道吸收脂肪来调节脂肪度

Seiichiro Ohgaku¹, Shogo Ida¹, Natsuko Ohashi^1,2

¹Department of Medicine, Shiga University of Medical Science, Otsu 520-2192, Japan.

|August 12, 2024

概括

内皮O-GlcNAcylation对于肠道脂质吸收至关重要. 破坏O-GlcNAcylation通过改变乳细胞结合,损害脂质吸收,提供新的肥胖治疗见解.

科学领域:

生物化学生物化学
细胞生物学细胞生物学
代谢过程中的代谢.

背景情况:

内皮细胞是营养物质运输的关键.
由OGT调节的O-GlcNAcylation充当细胞内营养传感器.
在内皮细胞中O-GlcNAcylation的功能尚不清楚.

研究的目的:

研究O-GlcNAcylation在内皮细胞中的作用.
确定内皮O-GlcNAcylation对营养代谢,特别是脂质吸收的影响.

主要方法:

生成的内皮细胞特异性Ogt-knockout (Ogt-ECKO) 的小鼠.
向Ogt-ECKO和对照小鼠提供正常或高脂肪饮食.
评估体重,葡萄糖和脂质代谢,以及肠道乳糖内皮细胞形态.

主要成果:

在高脂肪饮食中,Ogt-ECKO小鼠的体重减少和脂质吸收受损.
乳腺内皮细胞结点从按形状变为拉链形状的配置.
VEGFR3表达减少,但氧化供体的使用恢复了脂质吸收和细胞形态.

结论:

内皮O-GlcNAcylation通过乳母结形态学严格调节肠道脂质的吸收.
这项研究为代谢调节和潜在的肥胖治疗策略提供了新的见解.

关键词:

内皮细胞内皮细胞的内皮细胞.脂质的吸收脂质的吸收氧化氧化是什么?这是一种O-GlcNAcylation.肥胖问题肥胖问题血管内皮生长因子受体3 血管内皮生长因子受体3

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