通过调节miR-200c-3pp,CircRELL1促进了骨关节炎的进展
HongZhi Ding1, HaiJu Chen2, LianRong Dou1
1Department of Orthopedic, Shanghai Songjiang District Central Hospital, Shanghai, 201699, China.
Heliyon
|August 12, 2024
概括
循环RELL1 (circRELL1) 在骨关节炎 (OA) 中被上调,促进了疾病的进展. 抑制circRELL1通过向miR-200c-3p来缓解软骨损伤和炎症,为OA提供了潜在的治疗策略.
科学领域:
- 分子生物学分子生物学
- 生物化学 生物化学
- 病理学 病理学 病理学
背景情况:
- 越来越多的证据将圆形RNA与骨关节炎 (OA) 病原发生联系起来.
- 循环RELL1 (circRELL1) 在OA中的特定作用仍然在很大程度上未被描述.
- 骨关节炎是一种退行性关节疾病,影响全球数百万人.
研究的目的:
- 为了研究circRELL1在骨关节炎的发病过程中的功能作用.
- 阐明circRELL1参与OA的分子机制.
- 探索circRELL1作为OA的潜在治疗点.
主要方法:
- 分析了人类OA和正常组织样本.
- 用于模仿OA的Murine Destabilized Medial Meniscus (DMM) 模型和LPS诱导的冠状细胞.
- 通过RT-qPCR和免疫阻塞量化CircRELL1,miR-200c-3p和TCF4的表达.
- 评估了细胞活力,细胞亡,细胞因子水平 (IL-1β,IL-6,TNF-α) 和细胞外基质 (ECM) 降解标志物.
- 双 luciferase 报告员和 RIP 测定证实了分子相互作用.
主要成果:
- 在OA组织,DMM小鼠和用LPS治疗的细胞中,CircRELL1的表达显著上调.
- 沉默circRELL1改善了软骨退化,降低了OARSI得分,并改善了冠状细胞活动.
- 在OA模型中,circRELL1抑制抑制了炎症反应,亡和ECM降解.
- 揭示了 circRELL1 准 miR-200c-3p 的机制,以调节 OA 的进展.
结论:
- CircRELL1促进了骨关节炎的进展.
- 环RELL1/miR-200c-3p轴在OA的发病过程中起着至关重要的作用.
- circRELL1代表了骨关节炎治疗的潜在治疗标.
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