相关实验视频

Updated: Jun 17, 2025

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

Published on: March 28, 2018

通过STING介导的IL-6通过TCF4信号通路在胆固醇中抑制OATP1B1表达

Yan Guo^1,2, Hongjia Zhang², Nan Zhao²

¹Department of Endocrinology, Xiangya Hospital, Central South University, Changsha, Hunan, China.

Journal of clinical and translational hepatology

|August 12, 2024

概括

介质素-6 (IL-6) 通过降低β-catenin/TCF4.4的调节来抑制有机离子载体聚1B1 (OATP1B1) 的表达. 这种STING介导的IL-6通路可能会改变胆固醇性疾病中的药物药理动力学.

科学领域:

肝病学和药理学肝病学和药理学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

有机阴离子载体多 (OATPs) 对于输送胆酸和胆红素至关重要.
之前已经证明,介质素-6 (IL-6) 在胆固醇中降低了OATP1B3,但其对OATP1B1的影响仍然不清楚.
这项研究研究了IL-6对OATP1B1表达的影响及其在胆固醇疾病中的潜在机制.

研究的目的:

为了确定IL-6是否在胆固醇状况下抑制OATP1B1表达.
阐明IL-6影响OATP1B1.1.的分子机制.
探索STING信号在调节炎症因子和OATP1B1.1.中的作用.

主要方法:

在使用RT-qPCR和ELISA的胆固醇性小鼠模型中研究了STING对炎症因素的信号效应.
通过RT-qPCR和Western Blot治疗TNF-α,IL-1β和IL-6的肝细胞癌细胞 (PLC/PRF/5) 中评估了OATP1B1表达.
检查了OATP1B1调节器TCF4表达和β-catenin/TCF4与OATP1B1的相互作用,使用siRNA在PLC/PRF/5和HepG2细胞中的淘汰.

主要成果:

在胆固醇性小鼠中,STING抑制降低了炎症因素,IL-6显示出最强的OATP1B1抑制.
IL-6降低了β-catenin/TCF4的调节,从而降低了OATP1B1的表达.
通过siRNA介导的β-catenin/TCF4的敲除逆转了IL-6对OATP1B1.1.1的抑制作用.

结论:

由STING诱导的IL-6上调可能会抑制OATP1B1的表达.
降低OATP1B1功能可能会损害胆酸和胆红素的运输.
这种机制可能会导致IL-6水平升高的疾病中的药理动力学变化.

关键词:

胆酸是什么意思胆酸是什么意思胆红素是什么?胆红素是什么胆固醇的形成胆固醇的形成在IL-6的研究中,IL-6被认为是IL-6的基因.炎症炎症是一种炎症.刺痛是一种刺痛.在TCF4CF4中使用.这是一种β-catenin.

更多相关视频

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis

Published on: February 10, 2015

Ileectomy-induced Bile Overaccumulation in Mouse Intestine

Ileectomy-induced Bile Overaccumulation in Mouse Intestine

Published on: August 21, 2017

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...