活体和热杀 Pediococcus pentosaceus TAP04141 的益生菌和免疫刺激作用
1Department of Food Science and Biotechnology, Gachon University, Seongnam, 13120 Republic of Korea.
Food science and biotechnology
|August 12, 2024
概括
Pediococcus pentosaceus TAP041有效地降低了糖和甲基糖. 这种益生菌菌株还表现出免疫刺激性质,表明其在功能性食品中的潜在用途.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 食品科学 食品科学 食品科学
背景情况:
- 益生菌提供健康益处,调节免疫反应.
- 某些细菌菌株可以减少有害的反应性碳类物种,如glyoxal和methylglyoxal.
- 了解益生菌的免疫调节作用对于开发功能性食品至关重要.
研究的目的:
- 为了识别和表征一种具有降低糖和益生菌性质的细菌菌株.
- 评估已识别的菌株对巨细胞的免疫调节作用.
- 探索这种菌株作为功能性食品添加剂中的免疫刺激剂的潜力.
主要方法:
- 16S rRNA基因测序用于细菌识别.
- 在体外测定益生菌特征 (耐受性,粘附性,聚合性).
- 巨细胞 (RAW264.7) 刺激试验用于评估氧化的产生,酶的激活和细胞因子的表达.
主要成果:
- 被鉴定为Pediococcus pentosaceus的TAP041菌株,显示出优异的glyoxal和methylglyoxal减少.
- TAP041表现出显著的益生菌特性,包括对酸,胆盐和酶的耐受性,以及肠道粘附和自动/凝聚能力.
- 活生生的和被热杀死的P. pentosaceus TAP041都诱导了氧化的产生,并调节了巨细胞中的炎症性细胞因子表达 (TNF-α,IL-1β,IL-6) 和信号通路 (MAPK).
结论:
- Pediococcus pentosaceus TAP041是一种有前途的益生菌,具有显著的低糖活性.
- 这种菌株对巨细胞具有强大的免疫刺激作用,其介于氧化和细胞因子调节.
- 活体和非活化形式的P. pentosaceus TAP041都具有作为功能性食品添加剂的潜力,用于免疫支持.
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