牛H5N1流感病毒与人类类型的酸受体结合不良
Jefferson J S Santos1, Shengyang Wang2, Ryan McBride2
1Department of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
bioRxiv : the preprint server for biology
|August 12, 2024
概括
牛中高致病性H5N1禽流感 (HPAI) 病毒主要与禽类受体结合,而不是人类受体. 进一步监测这些在牛中演变的H5N1病毒至关重要.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 流行病学 流行病学
背景情况:
- 高致病性H5N1禽流感 (HPAI) 病毒,特别是2.3.4.4b类,最近在美国乳牛中出现.
- 禽流感病毒通常与α2-3酸受体结合,而人类流感病毒更喜欢在上呼吸道的α2-6酸受体.
研究的目的:
- 为了研究在牛中发现的H5N1禽流感病毒的受体结合特性.
- 为了比较禽类和牛类H5N1血质素 (HA) 的结合特性.
主要方法:
- 从鸟类H5N1分离物和来自牛的H5N1分离物中分析血凝素 (HA) 片段.
- 利用两种不同的方法来评估甘氨酸受体的结合特异性.
主要成果:
- 类2.3.4.4b的禽类和牛类H5N1分离物都表现出有效地与α2-3酸结合的糖受体结合.
- 对于任何一种隔离物,都没有观察到可检测到与α2-6酸结合的甘氨酸受体的结合.
结论:
- 目前在牛群中流传的H5N1病毒的类别2.3.4.4b对人型呼吸道受体的结合较差.
- 持续评估受体结合特性是必不可少的,因为这些H5N1病毒在牛群中演变.
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