相关实验视频

Updated: Jun 17, 2025

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

作为一种分子工具,CRISPR-Cas13d可以在胚胎中实现向基因表达敲除

Minyoung Kim^1,2,3, Erica J Hutchins^1,2,3

¹Department of Cell and Tissue Biology, University of California San Francisco, San Francisco, CA, USA.

bioRxiv : the preprint server for biology

|August 12, 2024

概括

研究人员调整了CRISPR-Cas13d系统用于胚胎中的基因敲除,这是一个强大的发育生物学模型. 这种新工具有效地减少了PAX7的表达,扩大了实验可能性.

科学领域:

发育生物学发展生物学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

小胚胎是发育生物学研究的重要模型.
基因干扰对于理解胚胎发育至关重要.
在其他模型生物中,CRISPR-Cas13d在基因淘汰方面取得了成功.

研究的目的:

适应和验证CRISPR-Cas13d系统用于胚胎中的向基因敲除.
在相关的发育背景下证明CRISPR-Cas13d的有效性.
扩大用于鸟类模型的基因操纵工具包.

主要方法:

调整CRISPR-Cas13d系统,用于输送到胚胎中.
针对PAX7基因的制,这是一个早期的神经标记.
评估基因表达和功能性淘汰疗效.

主要成果:

在胚胎中成功实施CRISPR-Cas13d用于基因敲除.
有效地减少PAX7表达和相关功能.
与阻断翻译的形态生物相比,它具有可比的击倒效率.

结论:

CRISPR-Cas13d是一种可行的和有效的工具,用于胚胎中的基因敲除.
这个系统补充了现有的方法,如CRISPR-Cas9和morpholinos.
这种适应增强了小模型系统的实验多功能性.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?在Cas13d.一个漂亮的小.神经峰的神经峰

更多相关视频

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Efficient Production and Identification of CRISPR/Cas9-generated Gene Knockouts in the Model System Danio rerio

Published on: August 28, 2018

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

相关概念视频

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.