Jove
Visualize
联系我们

相关实验视频

Updated: Jun 17, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K

通过使用主动反3D跟踪来确定单个纳米粒子电泳流动性和捕捉.

Alexis Johnson1, Kevin D Welsher1

  • 1Department of Chemistry, Duke University, Durham, NC 27708, USA.

bioRxiv : the preprint server for biology
|August 12, 2024
PubMed
概括

这项研究引入了3D单分子活跃实时跟踪 (3D-SMART) 进行精确的纳米粒子表征. 该方法准确地测量了单个纳米粒子大小和电泳性移动性 (EM),克服了整体平均技术的局限性.

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

In situ polymer nanoparticle densitometry via real-time 3D single-particle tracking.

The Journal of chemical physics·2026
Same author

3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.

Viruses·2026
Same author

High-speed 3D single-virus tracking reveals actin-aided viral trafficking of SARS-CoV-2 on the plasma membrane.

bioRxiv : the preprint server for biology·2026
Same author

Super-Resolving Particle Diffusion Heterogeneity in Porous Hydrogels via High-Speed 3D Active-Feedback Single-Particle Tracking Microscopy.

Small (Weinheim an der Bergstrasse, Germany)·2025
Same author

Super-resolving particle diffusion heterogeneity in porous hydrogels via high-speed 3D active-feedback single-particle tracking microscopy.

bioRxiv : the preprint server for biology·2025
Same author

Hour-Long, Kilohertz Sampling Rate Three-Dimensional Single-Virus Tracking in Live Cells Enabled by StayGold Fluorescent Protein Fusions.

The journal of physical chemistry. B·2024
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

科学领域:

  • 纳米技术 纳米技术
  • 材料科学 材料科学 材料科学
  • 生物物理学的生物物理.

背景情况:

  • 纳米粒子 (NP) 在医学和工程方面至关重要,但目前的特征方法,如动态光散射 (DLS) 和电泳光散射 (ELS),对于异质群体缺乏精度.
  • 集成平均技术为多分散或复杂的NP系统提供了不准确的数据,阻碍了开发和应用.

研究的目的:

  • 开发和验证一种新的单纳米粒子特征技术,以准确确定尺寸和电泳性移动性 (EM).
  • 克服聚散和异质纳米粒子样本的批量表征方法的局限性.

主要方法:

  • 应用3D单分子主动实时跟踪 (3D-SMART) 以同时测量NP大小和每颗粒子的EM.
  • 使用主动反和振荡电场通过最大概率估计来确定单粒子电磁场.
  • 采用非动作轴的平均平方位移,以准确地确定尺寸.

主要成果:

  • 3D-SMART成功地根据尺寸和表面特征确定了单个聚乙烯NP的独特EM.
  • 单纳米粒子电磁波证明在区分NP制剂方面比扩散更精确,电荷数被确定为<30%的不确定性.
  • 观察到随着离子强度的增加而减少的EM,与散装方法一致,并证明了没有微流体的实时电动力学捕获.

结论:

  • 3D-SMART为单个纳米粒子的表征提供了一个强大的方法,提供精确的尺寸和EM测量.
  • 这种技术增强了区分和分析各种纳米粒子群体的能力.
  • 无微流体的方法使得在生物相关环境和活组织中探索NP行为成为可能.

更多相关视频

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

4.0K
Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

7.6K

相关实验视频

Last Updated: Jun 17, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

14.9K
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
13:30

Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics

Published on: February 18, 2022

4.0K
Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment
09:13

Plasmonic Trapping and Release of Nanoparticles in a Monitoring Environment

Published on: April 4, 2017

7.6K