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外虫调节Drosophila眼中的Notch信号,通过非apoptotic的caspase活动来调节

Sudershana Nair^1,2, Nicholas E Baker^1,3,4,5

¹Department of Genetics, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461.

bioRxiv : the preprint server for biology

|August 12, 2024

概括

多菌中的额外菌体 (Emc) 蛋白通过控制酶活性来调节发育决策. Emc突变导致酶激活,通过Notch信号通路影响细胞生长和眼睛发育.

科学领域:

发展生物学发展生物学
细胞生物学细胞生物学
遗传学是一个遗传学.

背景情况:

细胞命运决定通常由转录因子调节,DNA结合抑制剂 (Id) 蛋白调节这一过程.
众所周知,Drosophila Id蛋白质的额外宏菌 (Emc) 能够防止前神经bHLH转录因子指定神经细胞命运.

研究的目的:

调查Emc在发育决策中的作用,超出其与转录因子的已知功能.
探索Emc,酶活性和Drosophila的发育结果之间的联系.
了解EMC影响细胞生长和眼睛发育的分子机制.

主要方法:

在Drosophila眼睛和翅膀影像盘中对EMC突变的分析.
评估酶活性及其在发育缺陷中的作用.
研究Notch信号通路组件,包括Delta蛋白水平.
对基因表达的检查,特别是与死亡相关的亡抑制剂 (diap1) 基因.

主要成果:

Emc 阻止了 bHLH 蛋白 Daughterless (Da) 的同质化,保持了与死亡相关的亡抑制剂 (diap1) 基因表达.
emc突变导致酶激活,导致加速的形态遗传,R7光受体规范失败,以及眼睛中状细胞分化延迟.
由卡斯帕斯介导的高达尔塔蛋白水平调节了EMC突变克隆中的Notch信号传递.
在EMC突变翅膀形象性磁盘中,增长抑制也取决于酶活性.

结论:

在Drosophila发育过程中,Emc在调节酶活性方面发挥着关键作用.
抑制卡斯巴酶活性,即使在非亡细胞中,对于防止异常发育至关重要,特别是在多虫眼中.
通过对Delta的酶依赖调节,Emc对Notch信号的影响是其发育作用的关键机制.

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