作为Daloa,科特迪瓦的新型尿路病原体Priestia flexa:基因组测序的见解
Dého Aristide Gbégbé1, Okran Beyosse Christophe Kacou1, N'goran Parfait N'zi1,2
1Department of Biochemistry-Microbiology Jean Lorougnon Guédé University, Daloa, Côte d'Ivoire.
International journal of genomics
|August 12, 2024
概括
在科特迪瓦从尿路感染中分离出来的三种细菌菌株被确定为Priestia flexa. 一种菌株表现出抗生素耐药性和毒性基因,而其他菌株则没有,这突显了该物种内的基因组多样性.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 尿路感染 (UTI) 是常见的,需要研究致病性细菌病原体.
- 从临床环境中分离出来的细菌的基因组表征提供了对它们的遗传构成和潜在病原性的洞察.
- 了解细菌菌株的遗传多样性对于开发有效的治疗策略至关重要.
研究的目的:
- 在科特迪瓦从尿路感染中分离出来的三种细菌菌株进行基因组特征.
- 用比较基因组方法识别分离的细菌菌株的物种.
- 为了调查这些菌株中毒性和抗生素耐药性基因的存在.
主要方法:
- 从患者尿样中分离的细菌.
- 全基因组测序和组装.
- 基因组比较分析包括平均核酸标识 (ANI) 和DNA-DNA数字杂交 (dDDH).
- 基因组预测分析用于编码序列 (CDS),基因,毒性因子,抗生素耐药性基因和移动遗传元素 (MGE).
主要成果:
- 菌株21LM07,21LM367和21LM1136被识别为Priestia flexa. 这些菌株被确定为Priestia flexa.
- 基因组大小范围从4,007,501到8,624,538bp,含有不同的GC.
- 菌株21LM367具有两个β-乳酸/四环素抗性基因和九个毒性基因,与缺乏这些的菌株21LM07和21LM1136不同.
- 在所有菌株中都发现了MGE,在21LM367和21LM1136.6菌株中发现了携带tet(K) 耐药基因的等离子体.
结论:
- 孤立的尿路感染菌株属于Priestia flexa物种.
- 基因组分析显示,P. flexa菌株中毒性和抗生素耐药性基因含量存在显著差异.
- 菌株21LM367中存在耐药性和毒性基因表明,与其他研究的菌株相比,其病原性潜力更高.
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