在HepG2细胞中对双衍生物可能产生的毒性作用的比较in silico和in vitro评估
Aylin Balci-Ozyurt1,2, Anıl Yirun1,3, Deniz Arca Cakır1,4
1Hacettepe University, Faculty of Pharmacy, Department of Toxicology, Ankara, Turkey.
Toxicology research
|August 12, 2024
概括
双甲 (BPA),BPF和BPS可能会影响免疫反应,亡和DNA. 需要进一步的研究来了解这些BPA替代品的毒性作用.
科学领域:
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
背景情况:
- 双在塑料和树脂中普遍存在.
- 双A (BPA) 与副作用和低剂量毒性有关.
- 作为BPA的替代品,双F (BPF) 和双S (BPS) 的使用量有所增加.
研究的目的:
- 评估在HepG2细胞中对免疫和亡标记物和DNA损伤的双衍生物的in silico预测抑制度 (pIC50s).
- 比较BPA,BPF和BPS的亡性,遗传毒性和免疫毒性影响.
- 在低剂量时评估双毒性.
主要方法:
- 对于双衍生物的pIC50s的in silico预测.
- 在实验室中通过卡斯巴酶活性评估亡.
- 对DNA损伤标记物的测量 (8-OHdG,OGG1).
- 在HepG2细胞中的免疫标记物 (IL-4,IL-10,TGF-β,TNF-α) 的量化.
主要成果:
- 在分析表明,双可能会改变免疫/亡标志物和DNA在低剂量.
- 实验室研究表明,双衍生物在IC30度下影响免疫标记物.
- BPF和BPS显示出潜在的亡性免疫毒性作用.
结论:
- 所有测试的双都显示出免疫和DNA改变的潜力.
- BPF和BPS表现出额外的亡性免疫毒性作用.
- 进一步的体和体内研究是必要的替代双毒性评估.
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