呼吸挥发体是由肠道微生物群形成的
Ariel J Hernandez-Leyva1, Amalia Z Berna2, Yang Liu2
1Division of Allergy and Immunology, Department of Medicine and Center for Women's Infectious Disease Research, Washington University School of Medicine, St. Louis, MO, 63110, USA.
medRxiv : the preprint server for health sciences
|August 12, 2024
概括
呼吸中的挥发性有机化合物 (VOC) 反映了肠道微生物群的组成和代谢活动. 这项研究确定了微生物衍生的VOCs在呼吸中,为非侵入性诊断提供了潜力.
科学领域:
- 微生物学 微生物学
- 代谢学 代谢学 代谢学
- 宿主-微生物相互作用
背景情况:
- 肠道微生物群在健康和疾病中起着至关重要的作用,但基因组特征阻碍了快速诊断.
- 肠道微生物产生各种各样的代谢物,包括挥发性有机化合物 (VOC),可以在呼出的呼吸中找到.
研究的目的:
- 调查呼吸中的VOC是否可以作为肠道微生物群组成和功能的非侵入性生物标志物.
- 建立一种方法来分析与肠道微生物组相关的呼吸VOC.
主要方法:
- 在27名健康儿童中,比较了呼吸中挥发性微生物和便性肠道微生物.
- 开发并运用了一种方法来捕获和描述使用气体染色体质谱法 (GC-MS) 来捕获和分析 gnotobiotic 小鼠的呼出的气息 VOC.
- 在单一殖民小鼠的纯培养和体内生物中分析了肠道微生物产生的VOCs.
主要成果:
- 儿童呼吸中的VOC成分与他们的肠道微生物组概况相关.
- 鼠呼吸的VOC概况受到肠道微生物群组成的显著影响.
- 在实验室中产生的细菌VOC可以在殖民小鼠的呼吸中检测到.
结论:
- 呼吸中的VOC受到肠道细菌代谢和组成的直接影响.
- 这一发现支持了呼吸分析在非侵入性肠道微生物组评估方面的潜力.
- 在呼吸中发现的微生物衍生的VOC为了解宿主-微生物相互作用提供了新的途径.
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