从Funtumia elastica (Preuss) 获得的具有抗微生物活性的植物球细菌的表型和分子识别Stapf
Bolanle A Adeniyi1, Mercy Ogunlana1, Christopher O Igbokwe1
1Department of Pharmaceutical Microbiology, University of Ibadan, Nigeria.
Avicenna journal of medical biotechnology
|August 12, 2024
概括
来自Funtumia elastica植物圈细菌的代谢物显示出对人类病原体的抗微生物活性. 这一发现有可能从植物圈微生物群中开发新药.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
- 药物发现 药物发现 药物发现
背景情况:
- 与植物植物化学物质相比,关于来自植物界细菌菌群的代谢物存在有限的研究.
- 一些植物圈细菌通过amensality产生代谢物以求生存和物种间的竞争.
- 这项研究的重点是Funtumia elastica (FE) 的Phyllospheric Bacteria (PB) 的代谢物.
研究的目的:
- 为了评估从Funtumia elastica (FE) 中分离出来的Phyllospheric Bacteria (PB) 的代谢物的抗菌活性.
- 使用表型和分子方法识别菌群微生物群.
- 探索这些代谢物的药物发现潜力.
主要方法:
- 生物圈细菌 (PB) 通过超声波从Funtumia elastica (FE) 中分离出来.
- 从夜间培养物中提取代谢物,用于抗菌检测的无细胞超级生物.
- 细菌鉴定涉及DNA提取,16S rRNA基因放大,以及对NCBI基因库进行BLAST分析.
主要成果:
- 从FE植物圈中获得了21个细菌分离物 (7个是阳性,14个是阴性).
- 代谢物对病原体表现出不同的抗菌活性,例如黄金葡萄球菌,大肠杆菌,肺炎克莱布西拉,Trychophyton rubrum,Candida albicans和Microsporum canis.
- 对15个生物活性分离物的测序确定了四个属:肠杆菌,细菌,Pontoea和Staphylococcus.
结论:
- 来自FE植物圈的细菌产生对人类病原体具有对抗性 (杀伤性) 活性的代谢物.
- 这些发现凸显了从植物圈细菌代谢物中发现新药的巨大潜力.
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