莱普托特里克斯·奥克拉西亚 (Leptothrix ochracea) 基因组显示,在Fe (II) 和有机碳上有混合营养生长的潜力
Gracee K Tothero1,2,3, Rene L Hoover1,2,3, Ibrahim F Farag4
1Microbiology Graduate Program, University of Delaware, Newark, Delaware, USA.
Applied and environmental microbiology
|August 12, 2024
概括
形成铁的微生物Leptothrix ochracea可以使用铁氧化和有机碳作为能量,这表明湿地中的柔性混合营养生长.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物地质化学生物地质化学
背景情况:
- 莱普托希克斯 (Leptothrix ochracea) 在溪流和湿地中形成了独特的铁矿化地毯.
- 尽管L. ochracea是最早被描述的微生物之一,但它从未被分离出来,因此其生理学尚未解决.
- 关键的问题仍然是铁的氧化是否支持其能量/增长,以及它是否是自的,异的或混合的.
研究的目的:
- 解决关于Leptothrix ochracea的关键生理问题.
- 研究其代谢能力,包括从铁氧化和碳同化中节能.
- 了解其作为混合性铁氧化剂的生态作用.
主要方法:
- 从环境样本的元基因组中重建了九个高质量的Leptothrix ochracea基因组.
- 对参与铁氧化 (cyc2,mtoA),硫氧化 (Sox) 和碳固定 (RuBisCO) 的基因进行分析.
- 在 silico 石化测量代谢建模和metatranscriptomic分析以评估 in situ 的代谢活动.
主要成果:
- 基因组证实了来自铁和硫氧化的节能潜力.
- 有证据支持碳固定和利用有机碳来源 (糖,有机酸).
- 转基因组显示铁氧化,有氧呼吸和有机碳利用基因的高表达,支持混合营养的生长.
结论:
- 莱普托希克斯 (Leptothrix ochracea) 具有显著的代谢灵活性.
- 这种生物体在含有有机碳的,富含铁的湿地中作为混合性铁氧化剂壮成长.
- 它利用铁的氧化和有机化合物的能量和碳,驱动生物地球化学过程.
关键词:
莱普托里克斯 (Leptothrix ochracea) 是一个红色的植物.化学石质变性 (chemolithotrophy) 是一种化学物质.铁的微生物地毯.铁氧化细菌是一种氧化铁的细菌.混杂性变异性 (Mixotrophy) 是一种变异性.更多相关视频
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