主体GTPase Dynamin 2调节顶峰结结构,以控制Listeria monocytogenes的细胞间传播
Serena Tijoriwalla1, Thiloma Liyanage1, Thilina U B Herath1
1Department of Microbiology and Immunology, University of Otago, Dunedin, New Zealand.
Infection and immunity
|August 12, 2024
概括
李斯特菌使用基于actin的移动性在细胞之间传播. 动氨酸2和Tuba蛋白的相互作用通过维持细胞结的张力来控制细菌的传播,而Listeria使用InlC克服了这一点.
科学领域:
- 细胞生物学 细胞生物学
- 微生物学 微生物学
- 传染病 传染病 传染病
背景情况:
- 通过食物传播的病原体Listeria monocytogenes通过基于actin的移动性在宿主细胞之间传播.
- 细菌蛋白InlC促进L. monocytogenes通过与人类支架蛋白Tuba相互作用传播,减少皮层张力.
研究的目的:
- 为了研究人类GTPase Dynamin 2在L. monocytogenes细胞间传播中的作用.
- 阐明Dynamin 2, Tuba和细菌入侵机制之间的相互作用.
主要方法:
- 研究了Dynamin 2与Tuba的SH3域的关联.
- 利用了Dynamin 2和Tuba的遗传 (敲击,基因删除) 和药理抑制.
- 使用显微镜观察了感染和未感染细胞中的Dynamin 2局部化.
- 在各种突变菌株和条件下评估细菌的传播和突起形成.
主要成果:
- 动力素2与Tuba结合,并合作限制L. monocytogenes的传播.
- 抑制Dynamin 2或Tuba恢复了对∆inlC细菌菌株的正常传播.
- 动力素2和Tuba对于保持结点线性和皮质张力至关重要.
- L. monocytogenes InlC将Dynamin 2从细胞结处移除,从而对抗其功能.
结论:
- 迪纳明2和Tuba形成了一个复合体,限制了缺乏InlC的李斯特菌的细胞间传播.
- 野生类型的L. monocytogenes通过表达InlC来克服这种限制,这破坏了Dynamin 2-Tuba复合体.
- 这项研究揭示了一种新的宿主-病原体相互作用机制,涉及Dynamin 2和Tuba控制细菌传播.
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