相关实验视频

Updated: Jun 17, 2025

Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens

Isolation of CD 90+ Fibroblast/Myofibroblasts from Human Frozen Gastrointestinal Specimens

Published on: January 31, 2016

隔离人类胃肌肉自身的免疫细胞的协议,用于单细胞分析

Shefaa AlAsfoor¹, Cheryl E Bernard¹, Erik Jessen²

¹Division of Gastroenterology and Hepatology, Enteric Neuroscience Program, Mayo Clinic, Rochester, MN 55905, USA.

|August 12, 2024

概括

这项研究提供了一个详细的协议,将免疫细胞从人类胃肌肉中分离出来. 这种方法有助于理解免疫细胞在胃肠道 (GI) 健康和运动中的作用.

科学领域:

免疫学免疫学免疫学
胃肠病学胃肠病学
神经科学是一个神经科学.

背景情况:

胃肠 (GI) 肌肉自身中的免疫细胞对于肠道神经系统和平滑肌肉的维护至关重要.
了解GI免疫细胞多样性是阐明它们在GI运动中的作用的关键.

研究的目的:

介绍一个详细的协议,以隔离单个免疫细胞从人类的胃肌肉自身.
为了进一步研究这些免疫细胞的功能.

主要方法:

组织的保存,剖析和解离的胃肌肉本身.
CD45+免疫细胞的磁性细胞分类.
单细胞RNA测序 (scRNA-seq) 用于细胞分析.

主要成果:

建立了一个全面的协议,用于从人类胃肌肉本身中分离单个免疫细胞.
该协议有助于在这个特定的胃肠道层内对免疫细胞种群的表征.

结论:

这一协议对于推进肠道内的免疫细胞相互作用的研究至关重要.
进一步的研究可以利用这种方法来探索免疫细胞对肠道功能和疾病的贡献.

关键词:

细胞隔离细胞的隔离.基因组学就是基因组学.免疫学免疫学免疫学分子生物学分子生物学在RNA-seqqq.测序测序是指测序的时间.一个单细胞单细胞.细胞生物学细胞生物学

更多相关视频

Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes

Isolation of Murine Intestinal Mesenchyme Resulting in a High Yield of Telocytes

Published on: March 24, 2023

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori

Published on: November 12, 2015

相关概念视频

Overview Of Cell Separation And Isolation

Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.